葡萄籽原花青素对鼠伤寒沙门氏菌的抗诱变作用

目的: 观察葡萄( V itis vinif era) 籽原花青素的抗诱变作用。方法:以S almonella typhimurium TA97 、TA98 、TA100 、TA102为标准试验菌株进行Ames 试验。结果:在不加S9 条件下,原花青素能显著抑制Dexon 诱发的TA97 、TA98回复突变,叠氮钠诱发的TA100回复突变,丝裂霉素C 诱发的TA102回复突变;在不加S9 条件下,原花青素能显著抑制2-氨基芴诱发的TA97 、TA98及TA100回复突变,而对1 ,8-二羟基蒽醌诱发的TA102回复突变抑制作用较强。结论:葡萄籽原花青素对多种化学诱变剂 有抗诱变作用。

ANTIMUTAGENIC EFFECT OF PROCYANIDINS FROM VITIS VINIFERA SEED IN SALMONELLA TEST

Purpose: Antimutagenic effect of procyanidins from Vitis vinifera seed was studied. Methods: A series of histidine requiring strains TA\-\{97\}, TA\-\{98\}, TA\-\{100\} and TA\-\{102\} were used for antimutagenicity test. Several mutagenic agents such as Sodium Azide(NaN\-3), Mitomycine C(MMC), Dexon, 2 AF, 1,8 Dihydroxyanthrachinon were used to make mutagenic model and antimutagenic effects of procyanidins were observed. Every tester strain was divided into model group, spontaneous mutation group and drug groups. Results: The results showed that procyanidins inhibited mutagenic effects induced by the most of the model agents, but little inhibitory effect was observed on TA\-\{102\} reverse mutation induced by 1,8 Dihydroxyanthrachinon(+S\-9). Conclusion: Antimutagenesis is one of mechanism chemoprevention of cancer of procyanidins.