IGF-1及TNF-α对大鼠成骨肉瘤细胞增殖的调节作用 |
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引用本文: | 王玉兰,刘彬.IGF-1及TNF-α对大鼠成骨肉瘤细胞增殖的调节作用[J].河南医学研究,1998,7(1):38-40. |
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作者姓名: | 王玉兰 刘彬 |
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作者单位: | 开封医学高等专科学校生物化学教研室,洛阳医学高等专科学校生物化学教研室 |
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摘 要: | 目的:通过对大鼠成骨肉瘤UMR106细胞增殖状态及细胞周期变化的观察,探讨胰岛素样生长因子 1(IGF 1)及肿瘤坏死因子 α(TNF α)对该细胞增殖调节作用的机理。方法:将UMR106细胞培养于含10%小牛血清的MEM培养基中。细胞长满后换无血清培养72h,然后分别用生理剂量的IGF 1和TNF α刺激细胞12h,应用3H TdR参入、磺基罗丹明染色法和流式细胞技术对细胞增殖状态及细胞周期进行定量测定。结果:IGF 1有明显促细胞DNA合成作用,并使S期细胞所占比例明显增加;而TNF α则具有相反的作用。定量测定细胞增殖也显示UMR106受IGF 1的正性调节而受TNF α的负性调节。结论:IGF 1和TNF α对UMR106细胞增殖的调节作用与该细胞DNA复制水平调节有关。
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关 键 词: | 成骨肉瘤细胞株 胰岛素样生长因子-1 肿瘤坏死因子-α 细胞周期 |
The Effect of IGF 1 and TNF α on the Regulator of Cell Proliforation in Osteoblist-like Cells |
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Abstract: | Objective: In this experiment rat osteoblastsarcoma cell line UMR 106,was used to study the effects of insulin-like growth factor 1 (IGF-1) and tumor necrosis factor (TNF-α) on the regulation of cell proliferation.Methods: UMR 106 cells were grown in MEM supplemented with 10% (v/v)FBS.After 48 h the cells were refed with serum-free medium.In there they were cultured for 72 h and treated with growth factor for 12 h. DNA synthesis was measured by using3H TdR incorporation.The cell number was measured by the SRB staining and the DNA cell-cycle analysis was measured by the flow cytometry(FCM).Results: The cells were treated with IGF-1(10-8mol/L).It was revealed that 3H TdR incorporation increased by 93.57% respectively,and the percentage of cells in S stage increased from 48.20% to 66.0%.In the case of TNF-α(10-8 mol/L) 3H TdR incorporation decreased by 57.51%,and the percentage of cells in S stage decreased from 49.30% to 22.60%.At the meantime,cell count were also increased in the definite period after IGF 1 treatment and decreased after TNF α treatment.Conclusion: In conclusion,these two kinds of growth factor are the potent effector on the cell proliferition in UMR 106. |
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Keywords: | osterblastsarcoma cell line insulin like growth factor 1 tumor necrosis factor α cell cycle |
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