叶酸干预对男性高脂血症者血清炎症因子的影响

目的探讨叶酸干预对男性高脂血症者单核细胞趋化因子-1（MCP-1）、可溶性血管细胞粘附分子-1（sVCAM-1）、白介素-6（IL-6）、白介素-8（IL-8）的影响。方法 2010年6—7月,由云南省云县人民医院体检筛查出120名男性高脂血症志愿者进行1︰1配对后随机分组,干预组给予叶酸2.5 mg/d,持续3个月,对照组不给予任何药物。干预前对所有参加者进行问卷调查,包括一般情况和流行病学调查,并采用膳食频率表对调查对象的饮食习惯、食物摄入量等进行膳食调查,同时进行体格检查,并检测血清MCP-1、sVCAM-1、IL-6、IL-8、叶酸含量。干预3个月后再次进行膳食调查、血清实验室检测。根据数据的不同情况采用相应的描述和统计方法。结果 120名男性高脂血症者年龄范围为30～61岁,已婚占97.5%（117/120）,大专及以上学历占69.2%（83/120）,少数民族占38.3%（46/120）。干预前,2组年龄及生理生化指标间的差异均无统计学意义（P〉0.05）。干预前干预组血清IL-8、MCP-1、sVCAM-1平均水平分别为219.02 pg/mL、334.69 pg/mL、130.16 ng/mL,在控制膳食不变的基础上,叶酸干预3个月后,干预组血清IL-8、MCP-1、sVCAM-1水平明显降低（分别为149.68 pg/mL、281.90 pg/mL、83.75 ng/mL）,与对照组（分别为243.16 pg/mL、342.95 pg/mL、130.26 ng/mL）间的差异有统计学意义（P〈0.05或P〈0.01）;干预组与对照组IL-6水平差异无统计学意义（P〉0.05）;血清叶酸浓度与IL-8、MCP-1、sVCAM-1呈负相关关系（r值分别为-0.419、-0.193、-0.409,P〈0.05或P〈0.01）,与IL-6关系不明显（P〉0.05）。结论叶酸对男性高脂血症人群可能有一定的抗炎作用。

Effect of folic acid on levels of serum inflammatory cytokines in men with hyperlipidemia.

Objective To test the effect of folic acid on levels of serum MCP - 1, sVCAM - 1, IL -6, and IL- 8 in men with hyperlipidemia. Methods A total of 120 male dyslipidemie volunteers were selected from physical examination in People＇s Hospital of Yunxian County, Yunnan Province. Subjects were randomly divided into intervention group and control group as 1 ： 1 paired blank - controlled trial. The man in the intervention group was given folic acid （2.5 rag/d） for 3 months, while the control group was not offered any drugs. At the beginning and after 3 months of the study, subjects were underwent phys- ical examinations, comprehensive questionnaire regarding their ordinary situation, daily habits and dietary situation, and blood samples were collected for assessing serum IL - 6, IL - 8, MCP - 1, sVCAM - 1, and folate. Proper statistical analyses were applied for different data. Results The ages of 120 subjects were 30 -61 years old. Of them, 97.5% （117/120） were married, 38.3% （46/120） were minority, and 69.2% （83/120） had an education level of college or higher. The differences of age and other biomarks before intervention were not statistically significant （ P 〉 0.05 ）. Before intervention, the serum concentra-tion of IL -8, MCP - 1, and sVCAM - 1 were 219.02 pg/mL, 334.69 pg/mL, and 130. 16 ng/mL, respectively. After 3 months of folic acid supplement with dietary situation controlled, levels of IL - 8, MCP - 1, and sVCAM - 1 were significantly decreased （ 149.68 pg/mL, 281.90 pg/mL, and 83.75 ng/mL re- spectively）, compared with the control group （243.16 pg/mL, 342.95 pg/mL, and 130.26 ng/mL, re- spectively） （ P 〈 0.05 or P 〈 0.01 ）. The change of IL - 6 was not statistically significant （ P 〉 0. 05 ）. There were relationships between serum folate and IL - 8, MCP - 1, and sVCAM - 1 （ r： -0.419, - 0. 193, and - 0.409, respectively, P 〈 0. 05 or P 〈 0. 01 ）. The relationship between serum folate and IL - 6 was not statistically significant （P 〉 0.05）. Conclusion Folic acid showed a direct antiinflammato- ry effect in men with hyperlipidemia.