大鼠脑室出血后脑铁代谢与脑积水发生关系的研究

目的 观察脑室出血后铁离子和铁蛋白的动态变化,及去铁敏治疗前后慢性脑积水的发生率.方法 SD雄性大鼠170只,随机分为5组:正常对照组、假性脑室出血组、脑室出血组、侧脑室注铁离子组及脑室出血加去铁敏组.以前肉后0.4 mm冠状面的侧脑室宽度评价脑积水的发生率,以亚铁嗪法测量大鼠脑脊液铁离子含量,以ELISA试剂盒测量脑组织铁蛋白含量.结果 正常对照组、假性脑室出血组无脑积水发生,脑室出血组、侧脑室注铁离子组、脑室出血加去铁敏组28 d时脑积水发生率分别为80％、73％、20％,脑室出血加去铁敏组脑积水发生率显著低于脑室出血组和侧脑室注铁离子组(P＜0.01).脑脊液中铁离子含量和脑组织中铁蛋白在脑室出血组和侧脑室注铁离子组中各个时相点均显著高于假性脑室出血组(P＜0.01);脑室出血加去铁敏组铁离子含量在各个时相点,及铁蛋白在7、28d时均显著低于脑室出血组(P＜0.01).结论 大鼠脑室出血后脑脊液和脑组织铁含量明显升高,去铁敏可降低脑脊液中铁离子和脑组织中铁蛋白含量,降低脑积水的发生率.

Research of correlation between iron metabolism in brain,tissue and occurrence of hydrocephalus after intraventricular hemorrhage in rats

Objective To observe the dynamic changes of ferric ion and ferritin after intraventricular hemorrhage （IVH）, and the incidence rate of chronic hydrocephalus before and after the treatment with deferoxamine. Methods The 170 male Sprague Dawley rats were divided randomly into 5 groups： normal control, sham-IVH, WH, ferric ion injection and deferoxamine treatment. The incidence rate of hydrocephalus was evaluated by the transverse diameter of the lateral cerebral ventricle 0.4 mm behind the bregma in the coronal plane. The ferric ion level in the cerebrospinal fluid （CSF） and ferritin in the brain tissue were measured by ferrozine method and ELISA respectively. Results No hydrocephalus occurred in normal control group and sham-IVH group, and incidence rate of hydrocephalus was 80%, 73% and 20% in IVH group, ferric ion injection group and deferoxamine treatment group respectively on day 28 postoperatively. The incidence rate of hydrocephalus was much lower in deferoxamine treatment group than in IVH group and ferric ion injection group （P〈0.01）. The ferric ion in the CSF and ferritin in the brain tissue in IVH group and ferric ion injection group were much higher than in sham-IVH group at every time point （P〈0.01）. The ferric ion concentration at every time point and ferritin concentration on day 7 and 28 were significantly lower in deferoxamine treatment group than in IVH group （P〈0.01）. Conclusions The ion contents in CSF and brain tissue increase obviously after IVH, and deferoxamine can decrease the incidence rate of hydrocephalus by reducing the contents of ferric ion in CSF and ferritin.in brain tissue.