微电极引导偏侧帕金森病猴脑深部电极植入方法的研究

目的 微电极记录(MER)联合MRI建立丘脑底核偏侧帕金森病猴脑深部电刺激(DBS)模型.方法 4只恒河猴在DSA下经一侧颈内动脉注射MPTP制作偏侧帕金森病模型,采用Kurlan Scale量表及阿扑吗啡实验评估模型效果.3.0T MRI扫描数据输入Leksell系统预定靶点坐标值,MER记录双侧丘脑底核电信号,确定靶点,植入电极并固定.术后MRI查看电极位置.结果 Kurlan Scale量表评分和阿扑吗啡实验表明:偏侧帕金森病模型建立成功.MER记录到两侧丘脑底核放电,且给药侧放电频率明显高于给药对侧(P＜0.01);其修正MRI预定坐标值,术后MRI复查电极末端均位于丘脑底核内.结论 MER是MRI显影不清时丘脑底核定位的必要补充,MER联合MRI可以成功建立定位精确的丘脑底核偏侧帕金森病猴DBS模型.

Research of methods for implantation of deep brain electrode under guidance from microelectrode recordings in monkeys with hemiparkinsouism

Objective To establish deep brain stimulation （DBS） model of the subthalamic nucleus （STN） in monkeys with hemiparkinsonism by microelectrode recording （MER） and MRI. Methods MPTP was injected into the unilateral internal carotid in 4 rhesus macaques under DSA to establish hemiparkinsonism model, and the model result was evaluated by Kurlan Scale and apomorphine test. The target coordinate was reserved by 3.0T MRI scan data input Leksell system. MER recorded electrical signal of bilateral STN and determined the target, then the electrode was implanted and fLxed according to the data of MER. The electrode position was checked by MRI postoperatively. Results Kurlan Scale scores and apomorphine test showed that the hemiparkinsonism model was established successfully. MER recorded the electrical discharge of bilateral STN, and the rate of electrical discharge at the side with MPTP injection was obviously higher than that at the other side （P〈0.01）. The reserved coordinate by MRI was revised by MER, and all the ends of electrodes were situated in the STN by MRI postoperatively. Conclusions MER is a necessary complement to locating the STN when the MRI image is obscure. DBS model of the STN in monkeys with hemiparkinsonism can be successfully established by MER combined with MRI.