嗜血细胞综合征患儿外周血Th17细胞的检测及意义

目的 观察嗜血细胞综合征(HPS)患儿外周血CD4+细胞白细胞介素(IL)-17的变化,探讨其在HPS发病中的作用及临床意义.方法 分离HPS患儿(初诊组和缓解组)和健康者(对照组)外周血单个核细胞(PBMC),采用免疫磁珠阴选CD4+T细胞,然后加佛波酯(PMA)、离子霉素(Ion),经固定、透膜处理进行细胞内染色,流式细胞术检测CD4+T细胞内IL-17+水平.结果 初诊组HPS患儿IL-17表达水平明显高于缓解组(t=11.34,P<0.01),二者又明显高于对照组(t=35.28、9.38,P<0.01).结论 Th17细胞水平与病情活动相关,是判断HPS患儿病情活动的指标之一.

Detection and significance of Th17 cells in peripheral blood from children with hemophagocytic syndrome

Objective To study the level and clinic significance of Th17 cells in peripheral blood from children with hemophagocytic syndrome(HPS).Methods Peripheral blood monouclear cells was separated in children with HPS(preliminary diagnosis group and relieved group) and healthy children(control group).CD4+T cells were negative selected by immunomagnetic beads,treated with phorbol ester(PMA),ionomycin(Ion),fixation and intra-cellular dyeing by penetrate membrane,and level of IL-17 in CD4+T cells were then detected by flow cytometry.Results Among children with HPS,The level of IL-17 in preliminary diagnosis group was significantly higher than that in relieved group(t=11.34,P<0.01),both of which were significantly higher than in control group(t=35.28,9.38,andP<0.01).Conclusion Th17 cells could play an important role in HPS.Level of Th17 cells might be one index for estimating disease status in children with HPS.