| 白色念珠菌体外生物膜形成与基因分型关系的研究 |
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| 引用本文： | 阳隽,张天托,朱家馨. 白色念珠菌体外生物膜形成与基因分型关系的研究[J]. 国际检验医学杂志, 2012, 33(13): 1539-1541 |
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| 作者姓名： | 阳隽 张天托 朱家馨 |
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| 作者单位： | 1. 广州医学院第二附属医院呼吸内科,广州,510260
2. 中山大学第三附属医院呼吸内科,广州,510630 |
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| 摘 要： | 目的 探讨白色念珠菌临床分离株体外生物膜形成与基因分型的可能关系.方法 选取52株呼吸道白色念珠菌分离株体外黏附生长24 h,用XTT减低法测定其增殖情况.采用重复序列PCR指纹技术分析白色念珠菌菌株基因类型,并进行聚类分析.结果 根据黏附生长的白色念珠菌增殖情况,有26株临床株形成生物膜能力"高",其余菌株形成生物膜能力"低".实验菌株的遗传相似系数为0.79±0.13,生物膜形成能力"高"及生物膜形成能力"低"的菌株间相似系数分别为0.8±0.14和0.81±0.12.结论 呼吸道白色念珠菌分离株的亲缘关系较近,但生物膜形成能力形似的菌株间未出现基因型聚集.
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| 关 键 词： | 念珠菌 白色 生物膜 聚合酶链反应 基因型 |
In vitro formation of biofilm by Candida albicans and its relationship with genotype |
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| Yang Jun , Zhang Tiantuo , Zhu Jiaxin. In vitro formation of biofilm by Candida albicans and its relationship with genotype[J]. International Journal of Laboratory Medicine, 2012, 33(13): 1539-1541 |
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| Authors: | Yang Jun Zhang Tiantuo Zhu Jiaxin |
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| Affiliation: | 1.Department of Respiratory Medicine,The Second Affiliated Hospital of Guangzhou Medical University,Guangzhou,Guangdong 510260,China;2.Department of Respiratory Medicine,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou,Guangdong 510630,China) |
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| Abstract: | Objective To study the relationship between in vitro formation of biofilm by Candida albicans(C.albicans) isolates and genotypes.Methods 52 strains of C.albicans isolated from respiratory tract were isolated and incubated for 24 h.The metabolic activities of cells within biofilm were measured by XTT-reduction assay.The genetic similarities of isolates were assessed by rep-polymerase chain reaction(rep-PCR) and analyzed by cluster analysis.Results 26 C.albicans isolates were with high performance of biofilm formation.Similarity coefficient of tested strains was 0.79±0.13.Similarity coefficient of isolates with high or low performance of biofilm formation were 0.8±0.14 and 0.81±0.12 respectively.Conclusion The relationship among C.albicans isolates from respiratory tract might be close.The isolates with similar ability to form biofilm might be without cluster of genotypes. |
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| Keywords: | Candida albicans biofilm polymerase chain reaction genotype |
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