Cyclin D1基因多态性和儿童急性淋巴细胞性白血病相关性研究

目的 探讨细胞周期素 D1(Cyclin D1)基因A870G多态性和儿童急性淋巴细胞白血病之间的相关性.方法 应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测118例急性淋巴细胞白血病患儿(实验组)和160例健康儿童(对照组)的Cyclin D1 A870G基因型,比较两组基因型频率和等位基因频率.结果 实验组基因型频率为AA型30.51%、AG型50%、GG型19.49%,对照组基因型频率为AA型20.63%、Aa型47.5%、GG型31.67%,差异均有统计学意义(P<0.05).实验组等位基因A、G的分布频率依次为55.51%、44.49%,对照组等位基因A、G的分布频率依次为44.38%、55.62%,差异均有统计学意义(P<0.05).B细胞型患儿基因型频率为AA型50%、AG型41.67%、GG型8.33%,T细胞型患儿基因型频率为AA型25.53%、AG型52.13%、GG型22.34%,差异有统计学意义(P<0.05).结论 Cyclin D1基因A870G位点多态性与儿童急性淋巴细胞白血病发病的易感性存在相关性.

Correlation between Cyclin D1 gene polymorphism and acute lymphoblastic leukemia in children

Objective To analyze the correlation between Cyclin D1 gene polymorphism and acute lymphoblastic leukemia(ALL)in children.Methods Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used to detect Cyclin D1 A870G genotype in 118 children with ALL(experiment group) and 160 healthy children(control group).Genotype and allele frequencies were compared between the two groups.Results The distribution frequencies of genotypes of AA,AG and GG of Cyclin D1 gene were 30.51%,50.00% and 19.49% in experiment group,and 20.63%,47.50% and 31.67% in control group,which were significantly different(P<0.05).The frequencies of allele A and G were 55.51% and 44.49% in experiment group,and 44.38% and 55.62% in control group with significant difference(P<0.05).The distribution frequencies of genotypes of AA,AG and GG of Cyclin D1 gene were 50.00%,41.67% and 8.33% in patients with B-cell type,and 25.53%,52.13% and 22.34% in patients with T-cell type with significant difference(P<0.05).Conclusion Cyclin D1 gene polymorphism might be related with the susceptibility to ALL in children.