1427例乙型肝炎患者PreS1、HBV DNA和HBV-M相关性分析

目的 探讨乙型肝炎患者前S1抗原( PreS1)、HBV DNA及乙型肝炎病毒标志物(HBV-M)的相关性,评价三者联合检测的临床意义.方法 对1 427例乙型肝炎患者分别使用实时荧光定量聚合酶链反应(FQ-PCR)检测血清标本中HBV DNA载量,采用酶联免疫吸附法(ELISA)检测HBV-M和PreS1水平.结果 HBsAg(+)、HBeAg(+)、HBcAb(+)乙型肝炎患者的PreS1、HBV DNA阳性率及病毒载量,均显著高于其他模式乙型肝炎患者,差异有统计学意义(P<0.05).HBeAg、PreS1和HBV DNA之间具有较高的符合率.结论 PreS1、HBV DNA和HBV-M单独检测均存在缺陷,三者联合检测对乙型肝炎患者的准确诊断、判断病毒复制情况和药物疗效的监测具有重要临床意义.

Correlation analysis of PreS1-Ag, HBV DNA and HBV-M in 1 427 patients with type B hepatitis

Objective To explore the correlation between Pre-S1 antigen(PreS1-Ag),hepatitis B virus(HBV) DNA and HBV markers(HBV-M) in patients with type B hepatitis,and evaluate the clinical value of combined detection of these three indexes.Methods HBV DNA was detected by real-time fluorescence quantitative polymerase chain reaction(FQ-PCR),PreS1-Ag and HBV-M were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared patients with other models of HBV-M,patients with HBsAg(+),HBeAg(+) and HBcAb(+) were with significantly higher PreS1-Ag positive rate and HBV DNA viral load(P<0.05).HBeAg,PreS1-Ag and HBV DNA were with relatively high coincidence.Conclusion There might be defection in separate test of HBV-M,PreS1-Ag and HBV DNA.Combined detection of theses three indexes might be with important clinical significance for the accurate diagnosis,judgment of viral replication and monitoring of therapeutic efficacy.