Lymphoblastoid cell lines and burkitt-lymphoma-derivee cell lines differ in the expression of a second epstein-barr virus encoded nuclear antigen |
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| Authors: | Ingemar Ernberg,Bengt Kallin,Joakim Dillner,Kerstin Falk,Barbro Ehlin-Henriksson,Marie-Louise Hammarskj ld,George Klein |
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| Affiliation: | Ingemar Ernberg,Bengt Kallin,Joakim Dillner,Kerstin Falk,Barbro Ehlin-Henriksson,Marie-Louise Hammarskjöld,George Klein |
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| Abstract: | Twenty-seven Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines and 27 EBV-carrying Bur-kitt-lymphoma-derived lines were analyzed for expression of the second EBV-encoded nuclear antigen (EBNA-2) by immunoblotting and anticomple-ment immunofluorescence with EBNA-2-specific sera. While all lymphoblastoid cell lines expressed EBNA-2, only 10 of the 27 BL lines were EBNA-2-positive. Comparison of the EBNA-2 coding Bam HI W-Y- and H-fragments of EBV-DNA in the different cell lines by restriction enzyme analysis suggests that EBNA-2 negativity is due either to sequence diversity or to a deletion in the BamHI WYH region. |
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