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| 牙齿形状调控相关基因Wnt6的克隆 | |
| 引用本文： | 郑梁,金岩,肖明振,轩昆.牙齿形状调控相关基因Wnt6的克隆[J].临床口腔医学杂志,2004,20(4):212-214. |
| 作者姓名： | 郑梁 金岩 肖明振 轩昆 |
| 作者单位： | 1. 第四军医大学口腔医学院牙体病科 2. 第四军医大学口腔医学院组织病理学教研室 3. 第四军医大学口腔医学院儿童口腔科 |
| 基金项目： | 第四军医大学创新基金重点课题资助 (CXO2F0 0 2 ) |
| 摘 要： | 目的 :获得新生猪牙胚的差异表达基因。方法 :利用消减杂交方法获得新生猪前后恒牙胚的差异表达基因 ,并通过反向点杂交排除假阳性 ,尔后进行测序定性分析。结果 :通过消减杂交获得Wnt6基因。结论 :Wnt6可能在决定牙齿形状发育的分子调控网络中起重要作用 |
| 关 键 词： | Wnt6 消减杂交 牙齿形状调控 |
| 文章编号： | 1003-1634(2004)04-0212-03 |
The study on cloning of the related gene Wnt6 regulating teeth patterning |
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| ZHENG Liang,JIN Yan,XIAO Ming-zhen,XUAN Kun.The study on cloning of the related gene Wnt6 regulating teeth patterning[J].Journal of Clinical Stomatology,2004,20(4):212-214. | |
| Authors: | ZHENG Liang JIN Yan XIAO Ming-zhen XUAN Kun |
| Institution: | ZHENG Liang,JIN Yan,XIAO Ming-zhen,XUAN Kun.Department of Operative Dentistry,College of Stamotology,Fourth Military Medical University,Xi'an 710032,China |
| Abstract: | Objective:To obtain the differentially expressed genes from subtractive gene library of pig incisor and molar patterning. Method: Differentially expressed gene library was obtained during pig incisor and molar patterning by modified subtractive hybridization technique, then negative genes were eliminated by reverse dot blot .Finally positive genes were sequenced and qualitatively analyzed. Result: the related gene Wnt6 regulating teeth patterning was got .Conclusion : Wnt6 could play the key role in the molecular system regulating teeth patterning. |
| Keywords: | Wnt6 modified subtractive hybridization technique teeth patterning |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |