淋巴结内淋巴细胞异质性荧光染色观察

淋巴细胞是有机体内功能极为复杂而形态相似的一个细胞系,它们是参与免疫反应的重要的细胞成分,并与自身免疫病的发生有密切关系。根据细胞膜表面有不同的表面抗原、受体和免疫球蛋白以及它们在免疫反应中的功能不同,可以把淋巴细胞分为T、B、K和NK细胞以及不同的亚群。但现有的形态学染色方法和显微镜技术尚不能有效地区分它们。我们用自己建立的淋巴细胞悬浮染色方法,以一种荧光色素硫代黄素显示了十种荧光颜色不同的淋巴细胞,并结合免疫学手段进一步证明了在免疫反应过程中,其中四种淋巴细胞(桔红色荧光核黄色荧光胞质,灰黄色荧光核淡蓝绿色荧光胞质,深蓝色荧光核鲜蓝色荧光胞质和蓝色荧光核淡蓝色荧光胞质的淋巴细胞)可以分化为与它们荧光颜色相同的浆细胞,它们是B细胞。有关其它六种荧光颜色的淋巴细胞类型有待进一步探讨。此外,对淋巴细胞的分类和关于不同荧光颜色的淋巴细胞是不同类型,还是淋巴细胞的不同的分化阶段,或者是淋巴细胞的不同功能状态,以及浆细胞的分化过程进行了讨论。

ON METACHROMATIC FLUORESCENT STAINING OF THE LYMPHOCYTES IN LYMPH NODES

Lymphocytes are a ceil line with functional complexity and morphological similarity in the body. They are important cell components taking part ia the immunologic reactions and bear intimate relationship with the genesis of autoimmune diseases. Lymphocytes can be subdivided into T,B,K and K N cells and different subpopulations on the basis of the presence of different surface antigens, receptors and immunoglobulins on the surface of cell membrane as well as their functional differences in the immunologic reactions. However, they can not be distinguished successfully by the existing morphological staining and microscopical techniques at present.Lymphocytes of ten different colors of fluorescence were demonstrated bylymphocyte suspension staining method established by us using a single fluo-rochrome thioflavine. With the use of immune-logical methods it was also shown that in the process of immunologic reactions four of them, i.e.,those with nucleus in orange-red and cytoplasm in yellow, nucleus in grayish yellow and cytoplasm in bluish green, nucleus in dark blue and cytoplasm in bright blue as well as nucleus in blue and cytoplasm in bluish fluorescence, could be differentiated into plasma cells being of identical colors of fluorescence with them, they are B cells. Further investigation is needed for the nature and classification of the 6 types of lymphocytes remained demonstrating different colors of fluorescence.The classification of lymphocytes, the problem whether the lymphocytes showing different colors of fluorescence are different types or different functional conditions in lymphocytes as well as the process of differentiation in plasma cells are discussed.