基质金属蛋白酶-2及基质金属蛋白酶-9的表达与胆囊癌转移、浸润的关系

目的 探讨胆囊癌组织中基质金属蛋白酶—2(MMP—2)及基质金属蛋白酶—9(MMP—9)的表达及其与胆囊癌浸润、转移的关系。方法 选取手术切除的胆囊癌标本42例及慢性胆囊炎标本15例，采用免疫组织化学的方法(SABC法)检测MMP—2及MMP—9蛋白的表达。并结合临床病理学指标进行统计学处理分析。结果 胆囊癌组织中MMP—2及MMP—9的阳性表达率显高于慢性胆囊炎。低分化及末分化组MMP—2的阳性率显性高于高分化组及中分化组。浆膜层浸润组、淋巴结转移组及肝脏转移组MMP—2的阳性率分别显性高于无浆膜层浸润组、无淋巴结转移组及无肝脏转移组。浆膜层浸润组及淋巴结转移组MMP—9的阳性率显高于无浆膜层浸润组及无淋巴结转移组。胆囊癌组织中MMP—2和MMP—9的表达有显的相关性。(P＜0．05)．二同时阳性表达组其浆膜层浸润、淋巴结和肝脏转移发生率显高于其中之一或均阴性组(P＜0．05)。结论 MMP—2的表达与胆囊癌的分化、浸润、淋巴结及肝脏转移密切相关。MMP—9的表达与胆囊癌浸润及淋巴结转移密切相关。MMP—2和MMP—9在胆囊癌组织中表达正相关，在胆囊癌的侵袭和转移过程中起协同作用。

Expression of matrix metalloproteinase-2(MMP-2) and the relationship with invasion and metastasis in human gallbladder carcinoma

Objective To explore the relationship between the expression of MMP-2 & MMP-9 and invasion and metastasis of GBC. Methods Expression of MMP-2 and MMP-9 were assessed by immunohistochemical methods in 42 specimens of GBC and 15 of cholecystitis,and a statistical analysis was performed.Results Positive expression rate of MMP-9 in the patients with lymphatic metastasis or serosa invasion was significantly higher than that without lymphatic metastasis or serosa invasion.Expression of MMP-2 is positive correlated to that of MMP-9( P<0.05 ).Occurrence rate of serosa invasion,lymphatic metastasis or liver metastasis in the patients with MMP-2+/MMP-9+ was significantly higher than that with MMP-2+/MMP-9-,MMP-2-/MMP-9+ or MMP-2-2/MMP0-9-( P<0.05 ).Conclusion The expression of MMP-2 is closed related to the differentiation,invasion,lymphatic metastasis and liver metastasis.The expression of MMP-9 is closed related to the invasion and lymphatic metastasis. Expression of MMP-2 is positive correlated to that of MMP-9.There may be cooperating effect upon invasion and metastasis of GBC between MMP-2 and MMP-9.