玉米须总皂苷对大鼠糖尿病模型肾脏和胰脏超微结构的影响

目的:探讨玉米须总皂苷对链脲佐菌素所致大鼠糖尿病模型肾脏和胰脏超微结构的影响。方法:采用尾静脉注射链脲佐菌素造大鼠糖尿病模型,通过观察肾脏、胰脏超微结构的改变,探讨玉米须总皂苷不同剂量(200,100,50 mg.kg^-1)对该模型大鼠血糖及肾脏和胰脏组织的影响。结果:采用尾静脉注射STZ的方法成功复制出大鼠糖尿病模型。与模型组比,高、中剂量玉米须总皂苷和二甲双胍均可显著降低第30天血糖(P<0.01);各剂量玉米须总皂苷和二甲双呱均显著对抗链脲佐菌素对胰岛细胞的损伤;二甲双胍、高、中剂量玉米须总皂苷均可显著升高肾小管上皮细胞线粒体体密度(Vv)、比膜面(δm)、常染色质Vv(P<0.01),均可显著降低比表面(δ)、核δ、异染色质Vv(P<0.01);低剂量玉米须总皂苷可明显增加近曲小管上皮细胞Vv(P<0.05)。结论:玉米须总皂苷有较好的降糖作用,对链脲佐菌素致糖尿病大鼠模型所引起的肾脏、胰脏损伤有较好保护作用。

Influence of Zea mays L.saponin(ZMLS) on ultrastructure of kidney and pancreas in diabetes rats induced by streptozocin.

OBJECTIVE: To discuss the effect of Zea mays L. saponin (ZMLS) on ultrastructure of kidney and pancreas in the diabetes rats induced by streptozocin. METHOD: The diabetic rat model was established by injections of STZ, blood glucose, the ultrastructure of the kidney and pancreas were observed. Result: Compared with the model group, the large, middle-dose ZMLS groups and melbinum group could remarkably decrease the blood glucose (P < 0.01), the large, middle, small-dose ZMLS groups could remarkably prevent the pancreatic islet beta-cell from the injury induced by Streptozotocin. Melbinum and the large, middle-dose ZMLS groups could remarkably increase mitochondrial Vv, deltam and euchromatin Vv (P < 0.01), and significantly decrease the delta, Nucleus delta and heterochromatin Vv (P < 0.01). The small dose of ZMLS obviously increases mitochondrial Vv (P < 0.05). CONCLUSION: ZMLS showed good effect on decreasing blood glucose and protection action on the kidney and pancreas injury of induced by STZ.