T淋巴细胞亚群及其细胞因子在宫颈癌患者外周血中的变化及意义

目的研究T淋巴细胞亚群在宫颈癌患者外周血的表达。方法分别选取2017年1月-2018年6月本院收治的宫颈癌患者24例(UCC组)、同期妇科门诊的宫颈上皮内瘤变患者28例(CIN组)、HPV感染患者32例(HPV组)以及健康体检对照组20例(HC组)为研究对象。采用流式细胞术检测4组患者外周血单个核细胞Th1、Th2、Th17、Treg细胞的百分率,ELISA法检测相关细胞因子IL-4、IL-17和TGF-β的表达水平。结果与CIN组、HPV组和HC组比较,UCC组外周血的Th2细胞百分比、Th17细胞百分比、Treg细胞百分比和Th17/Treg比值明显上升,而Th1细胞百分比和Th1/Th2比值明显下降,差异有统计学意义(P<0.05)。HPV组和HC组比较,差异无统计学意义(P>0.05)。与CIN组、HPV组和HC组比较,UCC患者外周血的IL-4、IL-17和TGF-β细胞因子表达水平明显上升,差异有统计学意义(P<0.05)。HPV组和HC组比较,差异无统计学意义(P>0.05)。结论Th1/Th2和Th17/Treg的免疫失衡参与了宫颈上皮内瘤变向宫颈癌的发生、发展过程。

Changes and significance of T lymphocyte subsets and their cytokines in peripheral blood of patients with cervical cancer

Objective To study the expression of T lymphocyte subsets in peripheral blood of patients with cervical cancer.Methods Twenty-four patients with cervical cancer(UCC group)admitted to our hospital from January 2017 to June 2018,28 patients with cervical intraepithelial neoplasia in the gynecological clinic(CIN group),32 patients with HPV infection(HPV group),and 20 healthy controls(HC group)were selected into this study.The percentages of Th1,Th2,Th17 and Treg cells in peripheral blood mononuclear cells of four groups were detected by flow cytometer.The levels of related cytokines IL-4,IL-17 and TGF-βwere detected by ELISA.Results Compared with CIN group,HPV group and HC group,the Th2 cells,Th17 cells,Treg cells and Th17/Treg ratio in peripheral blood of UCC patients increased significantly,while the Th1 cells and Th1/Th2 ratios decreased significantly,with the differences statistically significant(P<0.05).There was no statistical significance on the difference between HPV group and HC group,with the differences not statistically significant(P>0.05).Compared with CIN group,HPV group and HC group,the levels of IL-4,IL-17 and TGF-βcytokines in peripheral blood of UCC patients increased significantly,with the differences statistically significant(P<0.05).There was no significant difference between HPV group and HC group,with the differences not statistically significant(P>0.05).Conclusion The immune imbalance of Th1/Th2 and Th17/Treg is involved in the occurrence and development of cervical intraepithelial neoplasia to cervical cancer.