重组人促血小板生成素对人肝细胞株L02增殖的影响

目的探究重组人促血小板生成素（rhTPO）对健康人肝细胞株L02的影响。 方法以健康人肝细胞株L02为研究对象，通过不同剂量rhTPO对照组（0 U rhTPO）、低剂量组（150 U rhTPO）及高剂量组（750 U rhTPO）]处理L02细胞，观察各组L02细胞的存活率。采用Western-blotting检测各组细胞A组（0 U rhTPO）、B组（15 U rhTPO）、C组（150 U rhTPO）及D组（1 500 U rhTPO）]的细胞外信号调节激酶（ERK）及其磷酸化产物（p-ERK）蛋白的表达水平，并采用荧光定量PCR检测ERK信使RNA（mRNA）表达水平。 结果各组L02细胞存活率的比较，差异有统计学意义（F = 8.843，P = 0.016）。进一步两两比较发现，低剂量组及高剂量组的存活率均显著高于对照组（107.9 ± 4.0）%、（112.0± 1.6）%、（100.0 ± 2.5）%]，且高剂量组细胞的存活率更高（P均< 0.05）。各组L02细胞间ERK蛋白及ERK mRNA表达水平的比较，差异均无统计学意义（F = 1.140，P = 0.392；F = 0.437，P = 0.733），而各组L02细胞间p-ERK蛋白表达水平的比较差异有统计学意义（F = 31.400，P < 0.001），且B组、C组及D组细胞的p-ERK蛋白表达水平均显著高于A组细胞（P均< 0.05），而B组、C组及D组细胞p-ERK蛋白表达水平两两比较，差异均无统计学意义（P均> 0.05）。 结论rhTPO可通过激活ERK促进L02细胞数量增殖。

Effect of recombinant human thrombopoietin on proliferation in human hepatic cell line L02

ObjectiveTo investigate the effect of recombinant human thrombopoietin (rhTPO) on healthy human hepatic cell line L02. MethodsThe survival rate of L02 cells was observed among a control group (0 U rhTPO), a low-dose group (150 U rhTPO) and a high-dose group (750 U rhTPO) according to different doses of rhTPO. The expression levels of extracellular signal regulated kinase (ERK) and phosphorylation-ERK (p-ERK) protein were detected by Western-blotting, and the expression level of ERK messenger RNA (mRNA) was examined by fluorogenic quantitative PCR in the L02 cells among group A (treated with 0 U rhTPO), group B (treated with 15 U rhTPO), group C (treated with 150 U rhTPO) and group D (treated with 1 500 U rhTPO). ResultsThe survival rate of L02 cells was significantly different among the three groups (F = 8.843, P = 0.016). It was much higher in the low-dose group and high-dose group than in the control group, and highest in the high-dose group (107.9 ± 4.0)%, (112.0 ± 1.6)%, (100.0 ± 2.5)%; all P < 0.05]. However, there were no statistically significant differences in the expression levels of ERK protein and ERK mRNA among the four groups (F = 1.140, P = 0.392; F = 0.437, P = 0.733). Besides, the expression level of p-ERK protein was significantly different among the four groups (F = 31.400, P < 0.001). It increased obviously in group B, group C and group D as compared with group A (all P < 0.05), while no significant differences were noted among group B, group C and group D (all P > 0.05). ConclusionrhTPO can promote the proliferation of L02 hepatocytes by activating ERK.