| Bx亚型1例的分子生物学鉴定 |
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| 引用本文： | Liu W,Li D,Liu C. Bx亚型1例的分子生物学鉴定[J]. 中南大学学报(医学版), 2011, 36(12): 1213-1216. DOI: 10.3969/j.issn.1672-7347.2011.12.015 |
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| 作者姓名： | Liu W Li D Liu C |
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| 作者单位： | 天津市第一中心医院输血科, 天津 300192 |
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| 摘 要： | 采用标准分析方法分析1例Bx亚型的血清学特征,再采用PCR-SSP技术做ABO初步基因分型,并对ABO基因第6外显子和第7外显子进行测序.该标本血清学检测结果:与抗B反应为无或1+,与抗AB反应为1+或2+,自身不凝集.PCR-SSP结果为Bx02/O2.ABO基因测序结果显示:B基因外显子6出现261G缺失,297为...
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| 关 键 词： | ABO血型 Bx亚型 等位基因 Bx02 |
Molecular genetic research of a case of Bx subgroup |
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| Liu Wei,Li Daihong,Liu Chun. Molecular genetic research of a case of Bx subgroup[J]. Journal of Central South University. Medical sciences, 2011, 36(12): 1213-1216. DOI: 10.3969/j.issn.1672-7347.2011.12.015 |
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| Authors: | Liu Wei Li Daihong Liu Chun |
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| Affiliation: | Department of Blood Transfusion, First Central Hospital of Tianjin, Tianjin 300192, China |
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| Abstract: | Serologic characters of Bx subtype were analyzed with standard Methods , PCR-SSP method was used for its genotype, and DNA sequences of 6 and 7 exons of ABO genes were analyzed by DNA sequencing. Serologically, the anti-B test was no or 1+, with anti-AB (1+ or 2+). The patient's auto antibodies were negative. PCR-SSP showed the patient was Bx02/O2. ABO genetic locus sequencing was 261delG in exon 6 and 297 was GG homozygous. The sample was a combination of blood group B and O2 because 297A> G, 526C> G, 657C> T, 703G> A, 796C> A, 803G> C, 905A> G, 930A> G shown by direct DNA sequencing and 803G> C, 905A> G shown by cloning DNA sequencing. The serological phenotype of the specimen was B and the genetic sequence was a combination of ABO*Bx.02.1.1 and ABO*O21.01.1.1. |
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| Keywords: | ABO subtype Bx subtype allele Bx02 |
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