Identification of estradiol in the ovaries of the silkworm, Bombyx mori |
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| Authors: | E Ohnishi M Ogiso K Wakabayashi Y Fujimoto N Ikekawa |
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| Institution: | 1. Biological Institute, Faculty of Science, Nagoya University, Nagoya 464, Japan;2. Institute of Endocrinology, Gunma University, Maebashi 371, Japan;3. Department of Chemistry, Faculty of Science, Tokyo Institute of Technology, Tokyo 152, Japan |
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| Abstract: | Estradiol was extracted and partially purified from the ovaries of the silkworm, Bombyx mori. Identification of estradiol was done by use of radioimmunoassay (RIA) and by gas chromatography-mass spectrometry (GC-MS) after derivatization into the ethyldimethylsilyl derivative. Concentration of estradiol in the ovaries was estimated to be 176 pg/g (RIA) and 63 pg/g (GC-MS). |
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| Keywords: | To whom all correspondence should be addressed |
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