人胰腺癌细胞株放射敏感性的体外研究

目的通过体外实验探讨人胰腺癌细胞株的放射敏感性。方法培养人胰腺癌细胞株SW1990、Capan-1、AsPC-1、MIAPaCa-2、PANC-1、P3，应用集落形成实验测定胰腺癌细胞在6MVX线不同剂量照射后的存活分数，计算各株细胞的放射生物学参数。结果胰腺癌细胞株MIAPaCa-2对放射治疗最敏感，SF2为0．388，而Capan-1对放射治疗最不敏感，SF2为0．758，MIAPaCa-2与As-PC-1、AsPC-1与SW1990之间的放射敏感性无显著差异，其余各株胰腺癌细胞之间存在显著差异。结论不同胰腺癌细胞株的放射敏感性存在差异。

Study on radiosensitivity of 6 pancreatic cancer cell lines in vitro

Objective To evaluate the radiosensitivity of 6 human pancreatic cancer cell lines in vitro. Methods Human pancreatic cancer cell lines SW1990, Capan-1, AsPC-1, MIAPaCa-2, PANC-1 and P3 were cultured. Their surviving fractions after irradiation using 6MV X-ray at the dose of 0-10 Gy were determined by clonogenic assay. Then their radiosensitivity parameters were calculated. Results The cell lines differed widely in their radiosensitivity (SF2=0.388-0.758). MIAPaCa-2 was most sensitive to X-ray while Capan-1 was most insensitive to it. There was no significant difference in radiosensitivity between MIAPaCa-2 and AsPC-1 as well as between AsPC-1 and SW1999. However, there was marked difference in the radiosensitivity between 2 cell lines of other groups. Conclusions Different cell lines have different radiosensitivities. Thus different radiotherapies should be employed in different patients with pancreatic cancer in clinical practice.