丙酸倍氯米松纳米微囊对哮喘豚鼠体内嗜酸性粒细胞及黏附分子的影响

目的 探讨丙酸倍氯米松（BDP）聚乳酸-聚乙酸（PLGA）纳米缓释微囊对哮喘豚鼠体内嗜酸性粒细胞、细胞表面黏附分子及可溶性细胞间黏附分子-1（sICAM-1）的影响，阐明其抑制嗜酸性粒细胞浸润的可能机制。方法 采用荧光酶标记法、间接ELISA法测定哮喘豚鼠体内黏附分子及血、肺泡灌洗液（BALF）中嗜酸性粒细胞计数。结果 哮喘豚鼠血浆及BALF中嗜酸性粒细胞计数、sICAM-1的OD值显著高于正常组（P〈0．01），微囊组每3d给药1次治疗后明显下降（P〈0．05），同普通制剂组每日给药相比，差异无显著性。荧光显微镜下BLAF细胞沉淀悬液中，BDP微囊组、普通制剂治疗组可见带有荧光染色的细胞大小相对均匀、分布稀疏，模型组中可见大量染色细胞，大小不均、分布密集。结论 BDP纳米微囊制剂每3d给药1次能显著降低血浆、BALF中可溶性黏附分子及细胞表面黏附分子的含量，从而对炎性细胞募集至气道产生抑制作用。

Influences of BDP nanocapsules on eosinophil and intercellular adhesion molecule in asthmatic guinea pigs

Objective] To investigate the influences of sustained releasing nanocapsule preparation of beclomethasone propionate(BDP) on eosinophil and intercellular adhesion molecule in asthmatic guinea pigs. Methods] The OD values of soluble intercellular adhesion-1(sICAM-1) were detected by indirect ELISA. Cellular surface adhesion molecule of cell-deposit in bronchoalveolar lavage fluid (BLAF) was observed by double-antigen fluorescence immunograpy and the number of eosinophils were counted simultaneously. Results] The OD values of sIACM-1 and eosinophils in asthmatic guinea pigs were significantly higher than those of the normal, which was significantly reduced after treatment of inhalation BDP-nanocapsule once every 3 days and BDP everyday(P <0.05). With fluorescent microscope observation, the cell expressed cellular surface adhesion molecule in BALF distributed sparsely with relatively equal size after treatment. Conclusions] Inhalation of BDP-PLGA nano-capsules once every 3 days can significantly reduce the content of sICMA-1 and cellular surface adhesion molecule in BLAF and plasma, which inhabits eosinophil recuitment.