去卵巢应激大鼠结肠组织5-羟色胺受体3表达及意义的探讨

目的: 研究不同卵巢激素水平对束缚应激刺激大鼠动物模型结肠组织5-羟色胺受体3(5-HT3R)的影响. 方法: 24只成年雌性SD大鼠,随机分为3组:假手术(Sham)组、去卵巢(OVX) 组、去卵巢加雌二醇(E2)和黄体酮(P)(OVX E2 P)组,每组8只. 4 wk后均予以束缚1 h应激刺激,观察大鼠排便和玻璃小球排出情况;并采用RT-PCR方法,检测3组大鼠结肠组织5-HT3R mRNA的表达. 结果: 去卵巢组较假手术组、去卵巢 雌二醇 黄体酮组大鼠排便量增加,玻璃小球排出时间缩短(P<0.01);去卵巢组与假手术组及去卵巢 雌二醇 黄体酮组比较,5-HT3R mRNA的表达明显增加(P<0.01);去卵巢 雌二醇 黄体酮组与假手术组比较,5-HT3R mRNA的表达减少(P<0.01). 结论: 卵巢激素在肠易激综合征(IBS)发病中对5-HT3R的调节方面有重要作用.

Expression and significance of 5-HT3 receptors in the ovariectomy restraint stress rats colon

AIM: To investigate the effects of ovarian hormone on the expression of 5 HT 3 receptors in the restraint stress induced dysfunction rats colon. METHODS: Twenty four female SD rats were randomly divided into three groups: Sham operation; ovariectomy (OVX) and ovariectomy with estrogen (E 2) and progesterone (P) replacement therapy (OVX+E 2+P). The rats were given 1 h restraint stress 4 week after operation. The effect of restraint stress induced on fecal pellets and the time of glass pellets output were observed; and the expression of 5 HT 3 receptors mRNA in the colon was studied by RT PCR. RESULTS: In contrast with the sham group and OVX+ E 2+ P group, OVX group showed increase in the fecal pellets output and decrease in the time of glass pellets output ( P <0.01); and the level of 5 HT 3 receptors mRNA in ovariectomied rats colon was significantly increased, the expression of 5 HT 3 receptors mRNA was decreased in the hormone replacement therapy ( P <0.01). CONCLUSION: These results indicate that ovarian hormone plays an important role in regulation 5 HT 3 receptors in irritable bowel syndrome.