响应面法优选清喉利咽口含片的提取工艺

目的:优化院内制剂清喉利咽口含片的提取工艺。方法:在预试验基础上,以绿原酸、总黄酮(木犀草素-7-O-葡萄糖苷、芦丁、甘草苷)质量浓度的综合评分为因变量,浸泡时间、加水量、提取时间为自变量,采用响应面法优选清喉利咽口含片的提取工艺。采用HPLC测定绿原酸和总黄酮(木犀草素-7-O-葡萄糖苷、芦丁、甘草苷)的含量,流动相乙腈(A)-0.5%磷酸水(B)梯度洗脱(0~12 min,9%A;12~15 min,9%~15%A;15~40 min,15%~45%A;40~50 min,45%~15%A;50~80min,15%A),绿原酸和黄酮类成分的检测波长分别为327,256 nm。结果:最佳提取工艺条件为浸泡时间89 min,液料比15.3∶1,提取时间136 min,提取数2次。绿原酸、木犀草素-7-O-葡萄糖苷、芦丁、甘草苷平均质量浓度分别为1 843.537,184.845,93.939,48.898 mg·L~(-1)。结论:建立的数学模型预测性好、稳定可行,可作为清喉利咽口含片的中试生产工艺。

Optimization of Extraction Process of Qinghou Liyan Buccal Tablets by Response Surface Methodology

Objective: To optimize extraction process of Qinghou Liyan buccal tablets,and provide references for its pilot production. Method: Based on pre-experiments,effects of soaking time,solvent dosage and extracting time on overall desibirability of contents of chlorogenic acid and totale flavonoids (included rutin,liquiritin and luteolin-7-O-glucoside) were investigated and forecasted by response surface metholodgy.HPLC was used to determine contents of chlorogenic acid and three flavonoids with mobile phase of acetonitrile-0.5% phosphoric acid for gradient elution and detection wavelengthes of 327 nm and 256 nm. Result: Optimal extraction process was as following:soaked 89 minutes and extracted twice with 15.3 times the amout of water for per time of 136 minutes.Concentrations of chlorogenic acid,luteolin-7-O-glucoside,rutin and liquiritin were 1 843.537,184.845,93.939,48.898 mg · L^-1. Conclusion: This opimized extraction technology is stable and feasible with well prediction,it provides references for pilot production of Qinghou Liyan buccal tablets.