异位内膜氧化应激及雌激素受体水平与盆腔痛关系的研究

摘要:目的 探讨异位内膜氧化应激 （OS） 及雌激素受体 （ER） 水平与盆腔痛的关系。方法 取轻、 中、 重度盆腔痛子宫内膜异位症 （EMs） 患者异位内膜作为轻度组 （29 例）、 中度组 （34 例） 及重度组 （26 例）， 取正常子宫内膜作为对照组 （30 例）。采用黄嘌呤氧化酶法检测和对比各组内膜中超氧化物歧化酶 （SOD） 水平； 采用硫代巴比妥酸比色法检测和对比各组内膜中丙二醛 （MDA） 水平； 采用免疫组化法检测和对比各组内膜中 ER 水平； 分析 3 组异位内膜中 MDA、 SOD 与 ER 水平相关性。结果 3 组异位内膜中 SOD 水平均低于对照组， MDA 及 ER 水平均高于对照组（均 P<0.05）； 3 组异位内膜中， 随疼痛程度增加， SOD 水平逐渐降低， MDA 及 ER 水平逐渐升高 （均 P<0.05）； 轻度组内膜中 MDA 水平与 ER 水平无相关性， 中度及重度组内膜中 MDA 水平与 ER 水平呈正相关 （P<0.05）， 3 组异位内膜中 SOD 水平与 ER 水平均无相关性。结论 异位内膜存在 OS 及 ER 过表达， 二者参与 EMs 盆腔痛的发生并发挥协同作用。

The relationship between oxidative stress and estrogen receptor levels in ectopic endometrium and pelvic pain

Abstract:Objective To discuss the role of oxidative stress (OS) and estrogen receptor (ER) levels in ectopic endome⁃ trium in patients with pelvic pain. Methods The ectopic endometrium was taken from patients with mild, moderate and se⁃ vere pelvic pain, and samples were divided into mild group (n=29), moderate group (n=34) and severe group (n=26). The nor⁃ mal samples of endometrium (n=30) were used as control group. Xanthine oxidase method was used to detect the level of su⁃ peroxide dismutase (SOD), thiobarbituric acid colorimetric method was used to detect the level of malondialdehyde (MDA) and immunohistochemistry was used to detect the level of estrogen receptor (ER). The correlation of MDA, SOD and ER lev⁃ els was analysed between three ectopic endometrium groups. Results The SOD levels were significantly lower and MDA and ER levels were significantly higher in three ectopic endometrium groups than those in control group (P <0.05). The SOD levels were decreased, while MDA and ER levels were gradually increased with increased pain intensity in three groups of ec⁃ topic endometrium (P < 0.05). There was no correlation between MDA level and ER level in mild group (P > 0.05). A posi⁃ tive correlation was found between MDA and ER levels in moderate and severe groups (P < 0.05). There was no relationship between SOD and ER levels in three ectopic endometrium groups (P > 0.05). Conclusion Over-expression of OS and ER presents in ectopic endometrium, and both participate in the occurrence of pelvic pain, and create synergies in pelvic pain of ectopic endometrium.