Determination of underivatized glucosamine in human plasma by high-performance liquid chromatography with electrochemical detection: Application to pharmacokinetic study |
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Authors: | E Pashkova A Pirogov A Bendryshev E Ivanaynen O Shpigun |
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Institution: | aMoscow State University, Analytical Chemistry Department, 119991, GSP-1, Moscow, Russia |
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Abstract: | A simple, rapid and sensitive high-performance liquid chromatography method with electrochemical detection was developed for the determination of glucosamine in human plasma. Plasma samples were analyzed after a simple two-step procedure of protein precipitation with subsequent dilution. The chromatographic separation was performed on a Carbopack™ column (3 mm × 150 mm) with a mobile phase consisting of water and 200 mM sodium hydroxide. Detection was performed electrochemically in a pulsed voltammetry mode. The limit of detection was 2.0 ng/ml, inter- and intra-day precision were less than 10%. The method was successfully applied to the investigation of the pharmacokinetics of glucosamine in healthy man volunteers. |
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Keywords: | Glucosamine Liquid chromatography Electrochemical detection Pharmacokinetics |
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