蕨麻对大鼠缺血心肌的保护作用及其血清差异蛋白表达的影响

【目的】研究蕨麻乙醇提取物对大鼠结扎冠状动脉所致急性心肌缺血损伤的保护作用及其差异蛋白质表达谱的影响。【方法】选取雄性SD大鼠随机分为手术对照组,模型组,盐酸地尔硫卓组(diltiazem,30 mg/kg),蕨麻乙醇提取物7.2、3.6、1.8 g/kg干预组。连续灌胃10 d后,结扎在体大鼠冠状动脉致急性心肌缺血损伤模型。缺血时间持续30 min。记录各处理组动物心电图,左心室收缩压LVSP,左心室舒张末期压LVEDP及左室内压最大变化速率(±dp/dt max);取血清检测乳酸脱氢酶(LDH)、肌酸激酶(CK)含量;用强阴离子交换芯片(SAX-2)及弱阳离子交换芯片(WCX-2)结合表面增强激光解析电离飞行时间质谱技术分析大鼠急性心肌缺血损伤后不同处理组血清中蛋白质表达谱的改变。【结果】冠状动脉结扎致心肌缺血后,心电图ST段出现抬高,蕨麻醇提物7.2和3.6 g/kg组显著抑制了急性心肌缺血损伤导致的大鼠心电图ST段升高(P<0.05)。蕨麻醇提物各剂量组的各项心功能指标[左心室收缩压LVSP,左心室舒张末期压LVEDP及±dp/dt均优于模型组(P<0.05)]。心肌缺血后,血清中LDH和CK含量显著升高,蕨麻醇提物各剂量组血清中LDH和CK均显著降低(P<0.05)。蛋白质芯片结果显示:WCX-2芯片共获得338个血清蛋白质峰;SAX-2芯片共获得348个血清蛋白质峰。缺血30 min,模型组质/荷(M/Z)比为4 972 Da的蛋白质相对含量明显高于对照组(P<0.01),蕨麻干预后,该蛋白质峰随药物浓度升高而降低,至高剂量组时降至对照组水平,呈现出明显的剂量依赖关系,提示4 972Da蛋白质可能是蕨麻抗心肌缺血损伤的药物作用靶点之一,有待进一步研究证实。【结论】蕨麻乙醇提取物能够显著保护急性心肌缺血所致心肌损伤,改善心脏功能。不同剂量蕨麻干预后,心肌损伤后异常表达的蛋白质(4 972 Da)剂量依赖性下降。

Effects of Potentilla anserina L.on acute ischemia myocardiac muscle and the differentially expressed proteins in serum in rats

【Objective】To study the effects of Potentilla anserina L.on acute ischemia myocardiac muscle and its differentially expressed proteins in rats.【Methods】Male Sprague-Dawley rats were randomly divided into control operation group and injury groups which were subjected to coronary occlusion lasting for 30min and then subdivided into model and diltiazem group,intervention groups of alcohol extract of Potentilla anserina L.（7.2 g/kg,3.6 g/kg,1.8 g/kg）.The ST segment change of electrocardiogram was observed after left anterior descending artery occlusion for 30min and the left ventricular systolic pressure（LVSP）,maximum positive and negative change in pressure（±dp/dt）,left ventricular and diastolic pressure（LVEDP） were recorded during ischemia in rat hearts. Activities of LDH,CK in serum were measured.The change of protein expression pattern in serum was monitored with weak cationic exchanger chips（WCX-2） and strong anion exchange chips（SAX-2） by surface-enhanced laser desorption ionization-time of flight-mass spectrometry（SELDI-TOF-MS）.【Results】Pretreatment with alcohol extract of Potentilla anserina L.（7.2 g/kg、3.6 g/kg）could significantly decrease the ST segment ascdending.Myocardial functional of ischemic rats were significantly improved in all doses of alcohol extract of Potentilla anserina L.（P 0.05）.The activities of LDH and CK（P0.05） in the serum were significantly decreased in rats pretreated with alcohol extract of Potentilla anserina L.338 protein peaks were detected on WCX-2 chips and 348 protein peaks were detected on SAX-2 chips in serum.The protein peak of 4972Da increased significantly was observed in ischemia model groups and was decreased dose-dependently in pretreatment groups and was reached to the level of control groups in 7.2 g/kg extract of Potentilla anserina L groups.【Conclusion】Alcohol extract of Potentilla anserina L.could improve the myocardial function after acute myocardial ischemia in rats.It was suggested that 4972Da protein may be one of a target of anti-myocardial ischemia for Potentilla anserina L.