子痫前期血管紧张素转换酶基因多态性与血管紧张素的相关性研究

目的探讨ACE基因I／D多态性与血浆PRA、AngⅡ的相关性。方法应用聚合酶链反应（PCR）和放射免疫法检测子痫前期患者及正常孕妇ACE基因I/D多态性的分布、血浆PRA、AngU水平。结果病理组ACE基因I/D多态性D等位基因分布频率高于对照组（P〈0．05）；血浆AngⅡ水平在病理组和对照组各基因型问无差异（P〉0．05）；病理组携带D等位基因者血浆PRA水平低于对照组愀0．01）；病例组内DD基因型血浆PRA水平低于ID基因型（P〈0．01）；ID基因型血浆PRA水平低于II基因型（P〈0．051；DD基因型血浆PRA水平低于Ⅱ基因型（P〈0．01）。结论（1）ACE基因I／D多态性与子痫前期的发病相关。（2）ACE基因I／D多态性与子痫前期患者血浆AngⅡ水平无关。（3）ACE基因的D等位基因与子痫前期患者血浆PRA水平相关．ACE基因I／D多态性可能通过改变血浆PRA水平参与子痫前期的病理过程。

Study of Angiotensin-converting enzyme gene polymorphism and angiotensin with Preeclampsia

Objective To probe ralationship between ACE I/D polymorphism and Plasma PRA, Ang II levels in preeclamp- sia. Methods The method of Polymerase chain reaction （PCR） and radioimmunoassay were conduceted to examina ACE gene polymorphism and plasma PRA, Ang Ⅱ levels. Results The distribution of ACE gcne polymorphism between the preeclampsia group and the control group is a statistically significant difference （P〈0.05）; the D allele had lower plasma PRA levels in the preeclampsia group than in the control （P〈0.01）; Plasma PRA levels with D allele in the preeclampsia group was lower than with the I allelc（P〈0.05）.We did not find significantly difference about plasma Ang Ⅱ levels between differernt ACE genotypes（P〉0.05）. Conclusion （1）ACE I/D polymorphism associated with preeclampsia.（2）ACE I/D polymorphism had no associated with plasma Ang Ⅱ levels in preeclampsia.（3）D allele of ACE I/D associated with the change of plasma PRA levels in preeclampsia.ACE I/D polymorphism may participate in the pathophysiology of preeclampsia by changing the levels of plasma PRA.