纤维蛋白原降解产物对大鼠主动脉血管细胞的作用及蛋白激酶C抑制剂的影响

目的：研究蛋白激酶C（PKC）抑制剂对纤维蛋白原降解产物（FFDP）引起的大鼠主动脉血管内皮细胞（RAEC）损伤的保护作用和平滑肌细胞（RASMC）增殖的抑制作用。方法：RAEC损伤以乳酸脱氢酶释放测定，RASMC增殖以结晶紫染色法测定。结果：FFDP能促进RAEC释放乳酸脱氢酶，诱导RASMC增殖；PKC抑制剂槲皮素、Ro 31-8220剂量依赖性地抑制FFDP对RAEC的损伤，并抑制FFDP诱导的RASMC增殖。结论：PKC抑制剂对FFDP引起的RAEC有保护作用，对RASMC增殖有抑制作用。

Effects of protein kinase C inhibitors on the injury and proliferation of rat aortic vascular cells induced by fibrin fibrinogen degradation products

Objective: To study the effects of protein kinase C (PKC) inhibitors on the injury and proliferation of rat aortic vascular cells induced by fibrin fibrinogen degradation products (FFDP). Methods: The injury of rat aortic vascular endothelial cells (RAEC) was determined by the lactic dehydrogenase (LDH) release and the proliferation of rat aortic vascular smooth muscle cells (RASMC) by crystal violet staining. Results: FFDP obviously increased the LDH release of RAEC and the proliferation of RASMC. Quercetin and Ro 31-8220 inhibit the effects of FFDP on rat aortic vascular cells in a concentration-related manner. Conclusion: PKC inhibitors exert protective effect on the injury and proliferation of rat aortic vascular cells induced by FFDP.