食管支架术后再狭窄组织中表皮生长因子及其受体的表达分析

目的 研究动物食管支架置入术后不同时间食管组织中表皮生长因子(EGF)、表皮生长因子受体(EGFR)的表达状况，以及与再狭窄形成的关系。方法 将16只食管支架术后再狭窄模型犬分为4组，分别于术后1、2、4、8周取材，另设立6只对照犬，所有样本采用免疫组织化学(SABC)及RT—PCR方法检测支架术后组织中EGF、EGFR的蛋白及mRNA表达。结果 支架术后1、2周食管组织中，EGF、EGFR的mRNA和蛋白均有明显表达，表达量较正常组织显著增强，几乎所有组织均不同程度的表达EGF、EGFR。4周时表达显著减弱，术后8周其表达基本消失。EGF的阳性细胞主要是巨噬细胞及部分血管内皮细胞与平滑肌细胞；EGFR的着色细胞主要是巨噬细胞、淋巴细胞及成纤维细胞。结论 食管支架术后，局部组织中淋巴细胞、巨噬细胞以及成纤维细胞增殖活性的变化与再狭窄的病理过程存在密切的关系，是重要的调节细胞。

The expression analysis of EGF and EGFR in esophageal restnosis tissue after stenting

Objective To study the expression of EGF and EGFR in restenosis tissue of animal e-sophagus after several times of stenting in esophagus, and their relation with esophageal restenosis . Methods Animal models of esophageal stenosis after stenting were made from 16 adult healthy dogs, and allocated into 4 groups to take restenosis tissue as experimental samples at week 1,2,4 and 8. In addition, other 6 normal dogs served as control. To study the expression of EGF and EGFR at protein and mRNA level in these samples by immunohistochemistry ( SABC) and RT-PCR. Results At week 1 and 2, EGF and EGFR clearly expressed, the expressing level increased greatly as compared with the normal controls, the expression of EGF and EGFR existed almost in all the tissues. At week 4 the expression lessened greatly, and finally at week 8 disappeared. The positive cells for EGF were mainly macrophages, some vascular endothelial cells and smooth muscle cells, the positive cells for EGFR were mainly macrophages, lymphocyte and Fb. Conclusion After esophageal stenting, lymphocyte, macrophage and Fb in the local tissues were closely related to restenosis, and served as the important regulatory cells in the pathogenesis of restenosis.