疏肝健脾方对化学性肝损伤小鼠的保护作用

目的研究疏肝健脾方（SGJP）对CCl4诱发的化学肝损伤小鼠的保护作用。方法将小鼠随机分为6组：正常对照组、模型组、疏肝健脾方低剂量组、疏肝健脾方中剂量组、疏肝健脾方高剂量组、阳性药（联苯双酯，BP）组，给药组灌胃给药，每天1次，连续灌胃10d，正常组和模型组用等量的溶媒，于末次给药8h后，除正常组外其余每组腹腔注射0．1％CCl4橄榄油溶液（0．1mL／lOg）复制小鼠化学性肝损伤模型，检测小鼠血清谷氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、肝脏指数、肝组织超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽（GSH）含量，HE染色观察肝组织形态学变化。结果疏肝健脾方中、高剂量组能显著抑制小鼠血清ALT、AST活性的升高（P〈0．01），抑制肝组织MDA含量升高（P〈0．05或P〈0．01），提高肝组织SOD、GSH活性（P〈0．05或P〈0．01），降低肝指数（P〈0．05或P〈0．01），减轻肝脏的病理损伤。结论疏肝健脾方对小鼠化学性肝损伤具有一定的保护作用。

Protective Effects of Shuganjianpifang on Chemical Liver Injury Mice

Objective To study the protective effect of shuganjianpifang （SGJP） on carbon tetrachloride -induced chemical liver injury. Methods The mice are randomly divided into six groups： The Control, The model, The SGJP （7.8,15.6,31.2 g/kg） ,The BP. The SGJP were given by avage, 1 times a day for 10 days. After being given drug for 8 hours, the Model were induced by injection of carbon 0. 1% tetrachloride -olive oil solution （0. 1 mL/10g） by intra- peritoneal injection. After being given drug for 10 days, the ALT and the AST were assayed by spectrophotometry. The SOD, MDA, GSH were examined by Colorimetric methods. The liver index were also examined. The histopathologic chan- ges of the hepatic tissues were observed by the HE stained method. Results The liver index, the ALT level, the AST level and the MDA level were decreased by SGJP （ 15.6,31.2 g/kg）. The SOD level and the GSH level were increased by SGJP. Moreover, the degree of the liver injury were also inhibited by SGJP. Conclusions SGJP is a significantly pro- tective drug in chemical liver injury mice.