健康人外周血单个核细胞诱导的NK细胞对脑胶质瘤杀伤作用的探讨

目的 对体外培养的NK细胞杀伤脑胶质瘤细胞进行探讨,为脑胶质瘤的免疫细胞疗法提供理论基础.方法 从4个健康人外周血提取单个核细胞,在KRATM培养环境下,诱导产生NK细胞和LAK细胞.分别用细胞计数法和流式细胞仪检测NK细胞增殖和细胞表面特异性标志CD3/CDl6/CD56,并用MTr法检测NK细胞及LAK细胞对脑胶质瘤细胞的杀伤.结果 经过体外培养,平均可得到7.93×109个以上细胞.和LAK细胞相比,NK细胞对脑胶质瘤细胞LNI8、LN229、T98G和U87MG的敏感性更高.结论 NK细胞能够对脑胶质瘤细胞产生有效免疫应答,为过继性免疫细胞治疗脑胶质瘤提供可能.

Cytotoxicity of Natural killer (NK) cells expanded from human peripheral blood monocytes(PBMC) against malignant cells in vitro

Objective To observe cytotoxicity of in vitro expanded NK cells in a panel of malignant glioma cells and investigate potentials of adoptive NK cell therapy for patients with malignant gliomas. Methods NK cells and lymphokine -activated killer(LAK) cells were obtained from PBMC of 4 healthy individuals and expanded with KRATM media. Cells proliferation was measured by cell counting and specific cell markers in-cluding CD3/CD16/CD56 on NK cells were confirmed by flow cytometry. MTT assays were employed in cy-totoxicity assays. Results The in vitro expansion rate of NK cells was up to 7.93 × 109 in total, the cytotox-icity is more effective with NK cells than that of LAK cells against all tested glioma cells, including LN18 LN229 、T98G and U87MG. Conclusion NK cells expanded from PBMC in vitro are effective killing cells a-gainst malignant glioma cells, this may imply their anti - cancer potential for adoptive cell therapy with ma-lignant gliomas.