胃十二指肠疾病患者中cagA阳性幽门螺杆菌的普遍易感性

明确cagA阳性幽门螺杆菌（H.pyloril)胃十二指肠疾病患者中的感染情况。方法：用聚合酶链反应（PCR）方法扩增从30例十二指肠球部溃疡、30例慢性胃炎和6例胃癌虱活检组织中分离培养出的H.pylori菌株的cagA基因片段，用酶联免疫吸附测定（ELISA）方法检测血清抗H.pylori抗体，用免疫印迹（Western blot)方法检测血清抗cagA抗体。结果：用PCR方法测得的cagA基因阳性率为：十二指肠球部溃疡70％（21／30），慢性胃炎73％（22／30），胃癌83％（5／6）。66份血清标本抗H.phlori抗体均为阳性。用Western blot方法测得的抗cagA抗体阳性率为：十二指肠球部溃疡83％（25／30），慢性胃炎77％（23／30），胃癌83％（5／6）。结论：在我国人群中，cagA基因不能作为消化性溃疡的标志物，cagA阳性H.pylori在消化性溃疡和慢性胃炎患者中存在普遍易感性。

Generalized Infection of cagA-positive Helicobacter pylori in Patients with Gastroduodenal Diseases

Background/Aims: To investigate the infectious status of cagA-positive Helicobacter Pylon (H. Pylon) strains in patients with duodenal ulcer (DU), chronic gastritis (CG) and gastric carcinoma (GC). Methods: 66 strains of H. Pylon from biopsied specimens were obtained from 30 patients with DU, 30 CG and 6 GC. The presence of cagA gene was examined by polymerase chain reaction (PCR), the serum anti-H. Pylon antibody was detected by enzyme-linked immunosorbent assay (ELISA), while the serum anti-CagA IgG was detected by Western blot analysis. Results: The cagA gene was detected in 70% (21/30) DU, 73% (22/30) CG and 83% (5/6) GC patients. All patients were seropositive for H. Pylon antibody IgG. Anti-CagA IgG was detected in 83% (25/30) DU, 77% (23/30) CG and 83% (5/6) GC patients. Conclusions: These results indicate that cagA cannot be used as a marker for DU among Chinese patients. The prevalence of cagA-positive H. Pylon in patients with CG is also high.