血管生成拟态与翼状胬肉进行期及静止期的相关性研究

目的：研究血管生成拟态与翼状胬肉发生发展的相关性，初步探讨其临床意义。
　　方法：选取20例正常眼的结膜组织及翼状胬肉静止期和进行期各50例标本行苏木素－伊红（ HE）染色、过碘酸雪夫（ PAS）染色、免疫组化CD34、VEGF染色及免疫组化CD31染色联合PAS双重染色观察翼状胬肉新生血管和血管生成拟态情况。
　　结果：CD34和VEGF在翼状胬肉组织中的表达均高于正常结膜组织（两者P＜0．05），并且两者在进行期翼状胬肉组织中的表达均高于静止期（两者P＜0．05）。 PAS染色血管拟态区网眼间隔染成紫红色，进行期和静止期出现血管生成拟态的例数分别是38例和11例，差异具有统计学意义（P＜0．05），相关性分析显示血管生成拟态与进行期翼状胬肉呈显著密切正相关（ Spearman’s相关系数r＝0．540＞0．5，P＝0．000）。
　　结论：翼状胬肉除了新生小血管形成增多外，血管生成拟态也是其血供方式之一，并且血管生成拟态与翼状胬肉的进展具有密切关系。

Correlation of vasculogenic mimicry in the aggressive and quiescent period of pterygium

AIM: To study the relationship between vasculogenic mimicry and human pterygium and to evaluate the clinical significance.

METHODS: Twenty samples of normal conjunctiva and the aggressive and quiescent period of pterygium of both 50 cases were studied under the light microscope by HE stain, Periodic acid-Schiff stain(PAS), immunohistochemistry staining with CD34, VEGF and immunohistochemistry combined with PAS stain.

RESULTS: The expression of CD34 and VEGF in pterygium was significantly higher than those of normal conjunctiva specimens(All P<0.05). The expression of CD34 and VEGF in aggressive cases was apparently higher than that in quiescent cases(All P<0.05). Vasculogenic mimicry was identified in 38 of 50 aggressivcases and 11 of 50 quiescent cases by PAS stain, with a statistically significant difference(P<0.05). Bivariate analysis showed that vasculogenic mimicry were positively correlated with aggressive cases(Spearman's correlation coefficient r=0.540>0.5, P=0.000).

CONCLIUSION: Neovascularization exists in pterygium and vascular mimicry is one of the blood supply pathways of the pterygium and play an very important role in the development of pterygium.