GCDFP-15在腋臭微创手术后残余大汗腺中的表达

目的：观察GCDFP-15在腋臭微创手术后大汗腺残余组织中的表达,判断手术是否彻底或吸刮过度的情况。方法：收集284例（双侧）腋臭微创手术刮出物标本,同时随机提取术后双侧腋窝0.2 cm×0.2 cm皮肤组织,用GCDFP-15进行免疫组织化学检测,判断微创术后大汗腺残留情况。结果：吸出物中均含有大量大汗腺并表达GCDFP-15,表达率100%,小汗腺不表达GCDFP-15;提取皮肤组织中有143例可见大汗腺导管,128例表达GCDFP-15（P〉0.05）,29例有可疑大汗腺残留,GCDFP-15标记3例为阳性（P〈0.01）。结论：GCDFP-15在腋臭微创手术后残余大汗腺中有特异表达,可作为判断微创手术效果的病理依据。

The Application of GCDFP-15 in Detecting the Residual Apocrine of Axillary Bromhidrosis in Microinvasive Surgery

Objective：To find out whether the operation is complete or excessive curetting exists in microinvasive surgery by observing the expression of GCDFP-15 in the residual apocrine of axillary bromhidrosis.Methods： The apocrine sweat glaud（ASG） samples sucked out of axillary in 284（bilateral） bromhidrosis microinvasive cases were collected and 0.2 cm×0.2 cm skin tissues were randomly taken from both sides after operation.GCDFP-15 expression was detected with immunohistochemical staining method,and the postoperative residual ASG status was analyzed.Results： All of the extracted substance contained a large number of ASG.All of them expressed GCDFP-15,and the expression rate was 100%.Eccrine sweat glands did not express GCDFP-15;Extracted ASG duct could be seen in skin tissue of 143 cases by using HE staining method,and 128 cases of them expressed GCDFP-15（P0.05）;Uncertain residual ASG could be seen in skin tissue of 29 cases by HE staining,and 3 of them were proved GCDFP-15 positive expression（P0.01）.Conclusions： GCDFP-15 has specific expression in axillary bromhidrosis ASG residual of microinvasive surgery,and it could be used as pathological basis for evaluation of operation results.