电针对糖尿病大鼠坐骨神经传导速度和神经超微结构的影响

目的 :观察固本通络电针法对实验性糖尿病周围神经病变的防治作用。方法 :大鼠分为正常组、电针组和模型对照组 ,运用链脲菌素造成大鼠实验性糖尿病模型 ,用桥式鼠架使大鼠在清醒、安静的自然状态下接受电针“肾俞”“环跳”穴 ,用神经电生理方法观察坐骨神经传导速度 ,用透射电镜观察神经纤维的超微结构。结果 :电针组与模型组相比 ,左、右两侧NCV均明显加快 (P <0 .0 5) ,潜伏期均明显缩短 (P <0 .0 5) ,但未达到正常组的水平 ;电针组有髓纤维髓鞘脱失的程度有所减轻 ,神经纤维内微血管内皮细胞肿胀程度较轻。结论 :固本通络电针法对实验性糖尿病周围神经病变具有一定的防治作用。

Effect of Electroacupuncture on the Conduction Velocity of Sciatic Nerve and Its Microstructure in Diabetes Rats

Objective: To observe the prevention and therapeutic effects of electroacupuncture (EA) in the treatment of peripheral nervous lesion in experimental diabetes mellitus (DM) rats. Methods:42 Wistar rats were randomized into normal group (n=13), EA group (n=15) and model group (n=14). DM model was established by intraperitoneal injection of 2% Streptozotocin (STZ). Bilateral “Shenshu”(BL 23) and “Huantiao”(GB 30) were punctured with filiform needles and stimulated electrically for 20 min, once every other day (4 weeks altogether). The conduction velocity of the sciatic nerve was determined with electrophysiological method under anesthesia (20% urethane 5 mL/kg) and microstructural changes of the nerve fibers were observed under transmission electron microscope. Results: In model group, the conduction velocity of the bilateral sciatic nerves decreased significantly and the latency of the evoked action potential prolonged compared with those of normal group (P< 0.001). In comparison with model group, the conduction velocity of the bilateral sciatic nerve increased significantly (P<0.05) and the latency shortened markedly (P<0.05). In comparison with model group, both the demyelination ratio of the myelinated nerve fibers and the swelling state (thickness) of the endothelial cells of the micro-vessels in EA group reduced significantly (P<0.001). Conclusion: EA is of favorable effect in improving peripheral nervous lesion in MD rats.