| 奈韦拉平对HeLa细胞端粒酶活性和细胞周期的影响 |
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| 引用本文： | 李宏捷,谢文利,朱江.奈韦拉平对HeLa细胞端粒酶活性和细胞周期的影响[J].天津医药,2007,35(11):840-841. |
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| 作者姓名： | 李宏捷 谢文利 朱江 |
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| 作者单位： | 1. 天津医科大学口腔医学院口腔病理教研室,300070
2. 武警医学院药理教研室 |
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| 摘 要： | 目的研究奈韦拉平(nevirapine,NVP)对HeLa细胞端粒酶活性和细胞周期的影响.方法采用TRAP-PCR-ELISA方法检测HeLa细胞在奈韦拉平作用后端粒酶活性的变化,用流式细胞仪分析细胞周期的改变.结果NVP作用后,HeLa细胞端粒酶活性明显抑制(P＜0.05),并且HeLa细胞的G2/M期细胞含量明显增高(P＜0.01).结论NVP对肿瘤细胞的端粒酶活性有抑制作用.
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| 关 键 词： | 奈韦拉平 端粒 未端转移酶 细胞系 肿瘤 细胞周期 流式细胞术 |
| 修稿时间： | 2007-06-07 |
The Effects of NVP on Telomerse Activity and Cells Phases in HeLa Tumor Cells |
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| LI Hongjie,XIE Wenli,ZHU Jiang.The Effects of NVP on Telomerse Activity and Cells Phases in HeLa Tumor Cells[J].Tianjin Medical Journal,2007,35(11):840-841. |
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| Authors: | LI Hongjie XIE Wenli ZHU Jiang |
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| Institution: | Department of 0ral Pathology,College of Stomatology,Tianjin Medical University,Tianjin 300070,China |
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| Abstract: | Objective: To study the effects of NVP on telomerase activity and cells phases in HeLa tumor cells. Methods: Telomerse activity was determined by TRAP-PCR-ELISA in untreated and treated HeLa tumor cells by NVP. Results: Telomerse activity of HeLa was significantly inhibited when treated with NVP(P < 0.05),cell cycle of treated HeLa tumor cells were significantly increased in G2/M phase(P < 0.01). Conclusion: NVP can effectively inhibit telom-erse activity of tumor cells. |
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| Keywords: | Nevirapine telomerase cell line tumor cell cycle flow cytometry |
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