| rhIGF-1对神经细胞缺血再灌注损伤的保护作用 |
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| 引用本文： | 刘国萍,郭洁,黄维惠,汪志云,梁东春.rhIGF-1对神经细胞缺血再灌注损伤的保护作用[J].天津医药,2007,35(9):675-677,723. |
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| 作者姓名： | 刘国萍 郭洁 黄维惠 汪志云 梁东春 |
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| 作者单位： | 1. 天津市第一中心医院神经内科,300192
2. 天津医科大学内分泌研究所 |
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| 摘 要： | 目的：研究重组人胰岛素样生长因子1（rhIGF-1）对缺血再灌注所致神经细胞损伤的保护作用。方法：自新生大鼠脑组织中分离培养原代神经细胞,^3H—TdR掺入法检测rhIGF-1对神经细胞增殖的影响。将所培养的细胞分为正常对照组、类缺血对照组及损伤后rhIGF-1干预组，对不同组别的细胞进行类缺血-复氧处理，流式细胞检测细胞凋亡情况。结果：rhIGF-1可明显促进原代培养的神经细胞增殖，并降低再灌注损伤所导致的细胞凋亡。结论：rhIGF-1对神经细胞缺血再灌注损伤具有保护及治疗作用。
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| 关 键 词： | 胰岛素样生长因子Ⅰ 神经元 细胞 培养的 再灌注损伤 细胞凋亡 |
| 修稿时间： | 2006-09-152006-10-24 |
Protective Effects of Recombinant Human IGF-1 on Neural Cell in Ischemia-Reperfusion Injury |
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| LIU Guoping,GUO Jie,HUANG Weihui,WANG Zhiyun,LIANG Dongchun.Protective Effects of Recombinant Human IGF-1 on Neural Cell in Ischemia-Reperfusion Injury[J].Tianjin Medical Journal,2007,35(9):675-677,723. |
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| Authors: | LIU Guoping GUO Jie HUANG Weihui WANG Zhiyun LIANG Dongchun |
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| Institution: | Department of Neurology, First Central Hospital of Tianjin, Tianjin 300192, China |
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| Abstract: | Objective: To study the protective effects of recombinant human IGF-1 (rhIGF-1) on neural cell in ischemia-reperfusion injury. Methods: Neural cells were separated from the brain of neonatal SD rats. The effects of rhIGF-1 on cell proliferation were detected by 3H-TdR incorporation method. Cells were randomly divided into 3 groups including normal group,hypoxia control group and rhIGF-1 treatment group. Ischemia-reperfusion was performed in hypoxia control group and rhIGF-1 treatment group. Apoptosis rate was detected by flow cytometry. Results: rhIGF-1 promoted the proliferation of primary cultured neurons and decreased the apoptosis rate caused by ischemia-reperfusion. Conclusion: rhIGF-1 has protective and therapeutic effects on neurons in ischemia-reperfusion injury. |
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| Keywords: | insulin-like growth factor I neurons ceils cultured reperfusion injury apoptosis |
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