通经草叶中粉背蕨酸的分离及含量测定

目的对通经草叶中的特征成分粉背蕨酸进行分离、纯化和结构鉴定,并采用HPLC法对通经草叶中粉背蕨酸的含量进行首次测定。方法色谱柱为Betasil-C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-体积分数为0.1%磷酸水溶液(体积比为85∶15),检测波长215 nm,流速1.0 mL.min-1,室温,采用外标法测定。结果粉背蕨酸质量浓度在2.475~49.50 mg.L-1内线性关系良好(r=0.999 6),方法精密度(RSD)为1.6%(n=6),加样回收率为98.2%~100.8%。结论该方法操作简便、分析速度快、重复性好、结果准确可靠,可用于通经草叶中粉背蕨酸的含量测定。

Isolation and determination of alepterolic acid in the frond of Aleuritopteris argentea

Objective To establish the method for isolation and determination of alepterolic acid in the frond of Aleuritopteris argentea. Methods The HPLC method was performed on a Betasil-C18 column (4.6 mm × 250 mm, 5 µm) at the ambient temperature, using a mixture of methanol and 0.1 % phosphoric acid solution （V∶V = 85∶15） as mobile phase. The detection wavelength was set at 215 nm. The flow rate was 1.0 mL·min-1. Results There was good linear relationship( A =1.078×107 ρ-1.119×102，r=0.999 6) between the concentrations and the peak areas of alepterolic acid in the range of 2.475-49.50 mg·L-1. The recoveries were in the range of 98.2 %-100.8 %, and the relative standard deviation was less than 1.6 % (n=6). Conclusion The results indicate that this method is accurate, rapid, simple and reproducible, and thus suitable for the analysis of alepterolic acid in the frond of Aleuritopteris argentea.