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共表达HPV16L1、L2、E6、E7蛋白的重组非复制型痘苗病毒构建及鉴定
引用本文:黄薇,田厚文,任皎,范江涛,赵莉,边涛,陆振华,阮力. 共表达HPV16L1、L2、E6、E7蛋白的重组非复制型痘苗病毒构建及鉴定[J]. 中华实验和临床病毒学杂志, 2005, 19(3): 240-243
作者姓名:黄薇  田厚文  任皎  范江涛  赵莉  边涛  陆振华  阮力
作者单位:1. 530021,南宁,广西医科大学肿瘤医院妇瘤科
2. 中国疾病预防控制中心病毒病预防控制所应急技术中心
基金项目:国家高技术研究发展计划863项目资助(2002AA216041)
摘    要:目的构建共表达人乳头瘤病毒16型(HPV16)L1、L2、E6、E7蛋白的非复制型重组痘苗病毒人用疫苗株。方法以痘苗病毒为载体、利用同源重组技术筛选共表达HPV16L1、L2、E6、E7蛋白的重组痘苗病毒并对其进行鉴定。结果该病毒在CEF细胞上连续传至第15代,经斑点杂交结果表明重组病毒基因组中有L1、L2、E6、E7基因插入;经WesternBlot检测,重组病毒能稳定表达HPV16L1、L2、E6、E7蛋白。结论非复制型重组痘苗病毒NTVJE6E7CKL1L2可作为预防和治疗HPV16相关肿瘤及其癌前病变候选疫苗。

关 键 词:乳头状瘤病毒    宫颈肿瘤  痘苗病毒  疫苗
收稿时间:2005-01-10
修稿时间:2005-01-10

Construction and identification of non-replication recombinant vaccinia virus co-expressing human papillomavirus type 16 L1/L2/E6/E7 proteins
HUANG Wei,TIAN Hou-wen,REN Jiao,FAN Jiang-tao,ZHAO Li,BIAN Tao,LU Zhen-hua,RUAN Li. Construction and identification of non-replication recombinant vaccinia virus co-expressing human papillomavirus type 16 L1/L2/E6/E7 proteins[J]. Chinese journal of experimental and clinical virology, 2005, 19(3): 240-243
Authors:HUANG Wei  TIAN Hou-wen  REN Jiao  FAN Jiang-tao  ZHAO Li  BIAN Tao  LU Zhen-hua  RUAN Li
Affiliation:Department of Gynecologic Oncology, The Affiliated Oncology Hospital, Guangxi Medical University, Nanning 530021, China. huangwei@csco.org.com
Abstract:Objective To generate a human papilomavirus (HPV16) prophylactic and therapeutic vaccine candidate for cervical cancer. Methods HPV16 major capsid protein L1 gene/minor capsid protein L2 gene and HPV16 early E6/E7 genes were inserted into a vaccinia virus expression vector. A strain of non-recombinant vaccinia virus containing the sequences was obtained through a homologous recombination and identified. Results DNA hybridization confirmed that the HPV16L1/L2/E6/E7 genes were integrated into vaccinia virus DNA. Western Blot result showed that full-length L1/L2/E6/E7 proteins were co-expressed in CEF cells infected with the recombinant virus. Conclusion NTVJE6E7CKL1L2 could be taken as a candidate of prophylactic and therapeutic vaccine for HPV-associated tumors and their precancerous transformations.
Keywords:Papillomavirus   human  Cervix neoplasms  Vaccinia virus  Vaccine
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