Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQa gene |
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| Authors: | Janet M. Jung Catherine T. Comey David B. Baer Bruce Budowlel |
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| Affiliation: | (1) Forensic Science Research and Training Center, FBI Academy, 22135 Quantico, VA, USA;(2) Florida Department of Law Enforcement, Orlando Regional Crime Laboratory, 32801 Orlando, FL, USA |
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| Abstract: | Summary A simple, practical approach for the extraction of PCR-amplifiable DNA for the HLA-DQa gene from bloodstains deposited on various substrates is described. DNA from bloodstains is purified using Chelex 100 ion-exchange resin and then amplified. If amplification is not achieved, the extract is washed through a Centricon 100 dialysis/concentration tube. If the second amplification of this extract produces a negative result, the extract is processed with Chelex 100 again. This approach has been found to be reliable, safe, efficient and economical. |
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| Keywords: | PCR DQa DNA extraction |
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