反相高效液相色谱法测定骨髓感染引流液中克林霉素的浓度

目的建立测定引流液中克林霉素含量的方法,探求骨髓感染抗菌药物载药系统克林霉素+半水硫酸钙骨水泥临床应用意义。方法采用Agilent ZORBAX Extend-C₁₈(150 mm×4.6 mm,5μm)为色谱柱;磷酸盐缓冲液(每1 mL中含磷酸二氢钾6.8 mg,用25%的氢氧化钾调节pH至7.5):乙腈=55∶45为流动相;检测波长为210 nm;流速为1.0 mL·min^-1。结果克林霉素在1.48~88.90μg·mL^-1范围内呈良好线性关系(r=0.9997),低、中、高浓度的平均回收率为92.90%、98.03%、97.13%;日内和日间相对标准偏差(RSD)分别小于2.0%和3.5%。结论反相高效液相色谱法测定引流液中克林霉素的浓度方法简便、灵敏、准确,对载克林霉素半水硫酸钙骨水泥临床应用有一定参考意义。

Determination of clindamycin in drainage fluid of bone marrow infection by HPLC

Objective To establish an HPLC method for determining clindamycin in drainage fluid and explore the clinical applicability of clindamycin hemihydrate calcium sulfate bone cement in the local antibacterial drug delivery system.Methods Agilent ZORBAX Extend-C₁₈(150 mm×4.6 mm,5μm)was used as the column.Phosphate buffer(6.8 mg·mL^(-1 )KH₂PO₄,pH adjusted to 7.5 with 25%KOH)and acetonitrile(55∶45)served as the mobile phase.The detection wavelength was 210 nm and the flow rate was 1.0 mL·min^-1.Results Clindamycin presented a good linear range of 1.48 to 88.90μg·mL^-1(r=0.9997).The average recoveries were 92.90%,98.03%and 97.13%respectively.The RSD values were less than 2.0%and 3.5%.Conclusion The reversed-phase high performance liquid chromatography method for the determination of concentrations of clindamycin in drainage fluid is simple,sensitive and accurate,which is of referential value for clinical applications of clindamycin semi-water calcium sulfate bone cement.