不同剂量黄芪对糖尿病大鼠肾脏氧化应激的抑制作用

  目的：研究不同剂量黄芪对链脲佐菌素诱导的糖尿病大鼠肾脏氧化应激的抑制作用  方法：40只健康雄性Wistar大鼠随机选取8只作为正常对照组,然后将剩余大鼠用链脲佐菌素诱导为糖尿病模型,并将建模成功大鼠随机分为糖尿病组,黄芪低、中、高剂量组。干预12周末检测各组大鼠糖化血红蛋白、24 h尿蛋白、肾功能等生化指标,大鼠尿液、血清及肾皮质中超氧化物歧化酶(SOD)活性、肾皮质丙二醛(MDA)及8-羟基脱氧鸟苷(8-OHdG)的含量,并对肾脏组织进行形态学观察  结果：与糖尿病组比较,黄芪各剂量组MDA及8-OHdG含量明显下降,SOD活性显著增强(P<0.05,P<0.01),肾功能指标明显改善(P<0.01),病理改变明显减轻；其中以高剂量组改善最为明显  结论：黄芪可减轻糖尿病大鼠肾脏氧化应激损伤程度,且存在明显的量效关系。

Inhibitory effects of different doses of Astragalus membranaceus on oxidative stress in the kidneys of diabetic rats

  Objective:To investigate the inhibitory effects of different doses of Astragalus membranceus on the oxidative stress in the kidneys of diabetic rats  Methods:Totally 40 healthy male Wistar rats were used, from which 8 rats were randomly selected as normal control group. Then the rest rats were induced to diabetic rat models by injections of streptozotocin, and the successful models were divided randomly into four groups:the diabetes mellitus group, the low dosage Astragalus membranceus (A_l) group, the middle dosage Astragalus membranceus (A_m) group and the high dosage Astragalus membranceus (A_h) group. After 12 weeks of intervention, the biochemical test was used to measure the levels of 24-hour urine protein quantitate, renal function, and other tests were done to measure the activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and 8-hydroxy-deoxyguanosine (8-OHdG) in the renal cortex  Results:The content of MDA and 8-OHdG in the Astragalus membranceus treatment groups were markedly lower and SOD activity significantly increased than those in the diabetes mellitus group (P<0.05, P<0.01), and renal function improved significantly (P<0.01), and pathological changes obviously alleviated; the Astragalus membranceus treatment high dose group improved most obviously than other groups  Conclusion:The traditional Chinese herbal medicine Astragalus membranceus could inhibit the oxidative stress in the kidneys of diabetic rats with an obvious dose-effect relationship.