四君子汤对衰老模型小鼠mtDNA缺失的影响

目的 探讨四君子汤对衰老模型小鼠mtDNA缺失的影响及其分子生物学作用机制.方法 取昆明种小鼠,按体重随机分为6组,即正常对照组,模型对照组,四君子汤高、中、低剂量组(灌胃20,10,5 g·kg-1)和维生素E组(灌胃250 mg·kg-1),每组20只,分笼饲养.以D-半乳糖法制备小鼠衰老模型,运用水迷宫实验,通过对小鼠空间辨别性学习能力进行训练来评估其学习记忆能力,观察四君子汤对衰老模型小鼠mtDNA(Mitochondrial DNA,线粒体DNA)缺失的影响.结果 模型对照组和四君子汤低剂量组(5 g·kg-1)小鼠海马mtDNA中存在着约3.87 kb片断缺失.四君子汤高、中剂量组(20,10 g·kg-1)未见缺失片断.结论 四君子汤具有防御模型小鼠脑mtDNA缺失突变的作用,可能是其延缓衰老的分子生物学作用机制.

Effect of Sijunzi Decoction on Mitochondrial DNA Missing of the Aging Model Mice

Objective To observe the effect of Sijunzi Decoction (SD) on the mitochondrial DNA deletion of the aging model mice, and to explore its anti-aging biomolecular mechanism. Methods Kunming mice were divided into 6 groups according to the body weight: normal control group, .model control group, high-, middle-and low-dose SD groups (at the dose of 20, 10, 5 g·kg-1 respectively), and vitamin E group (250 mg·kg-1), 20 mice in each group.The mice models of aging were induced by D-galactose. Water maze test was used to evaluate the learning and memory ability of the aging model mice. The effect of SD on the mitochondrial DNA deletion of the aging model mice was ob-served. Results In the hippocampal mtDNA of model control group and low-dose SD group, there were about 3.87 kb fragment missing. But there was no missing pieces in high-and middle-dose SD groups. Conclusion The anti-aging biomolecular mechanism of SD is probably related to the preventive effect on the mutation and deletion of the mitochondrial DNA in the aging model mice.