蜜环菌多糖对小鼠免疫功能的影响

蜜环菌多糖(100mg/kg·d×5d,ig)能显著增加正常小鼠和注射环磷酰胺所致免疫功能抑制小鼠的溶血素生成。当剂量为50mg/kg·d×5d,ig时,它还能显著增加正常小鼠的空斑形成细胞数。体外试验,蜜环菌多糖(10、50μg/ml)显著增强刀豆素A诱导的小鼠淋巴细胞增殖反应,但对二硝基氯苯所致小鼠迟发型过敏反应无显著增强作用。此外,蜜环菌多糖还能增加小鼠静注碳粒廓清速率及腹腔巨噬细胞的吞噬百分数和吞噬指数。

EFFECTS OF ARMILLARIELLA MELLEA POLYSACCHARIDE ON IMMUNOLOGCAL FUNCTION IN MICE

Armillariella mellea polysaccharide ( AP ) was isolated from solid fermented Armillariella mellea. AP ( 100 mg/kg·d, ig × 5 d ) increased the production of serum hemolysin in normal mice as well as in im-munodepressed mice induced by cyclophosphamide. At the dose of 50 mg/kg·d ig×5d, AP also caused a significant increase of spleen plaque forming cells ( PFC ) in normal mice. AP ( 10, 50μg/ml ) markedly enhanced Con A-induced lymphocyte proliferation of mouse spleen cells in vitro, but it had no potentiating effect on delayed cutaneous hypersensitivity ( DCH ) to 2,4-dinitrochlorobenzene ( DNCB ) in normal mice. AP ( 100 mg/kg/d, sc× 7d) increased both clearance rate of iv charcoal particles and phagocytic activity of ma-crophages of abdominal cavity in normal mice.