| 星形胶质细胞活化与表皮生长因子受体表达的相关性研究 |
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| 引用本文： | 郭志慧,武衡.星形胶质细胞活化与表皮生长因子受体表达的相关性研究[J].中风与神经疾病杂志,2010,27(8). |
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| 作者姓名： | 郭志慧 武衡 |
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| 作者单位： | 南华大学附属第一医院神经内科,湖南,衡阳421001 |
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| 摘 要： | 目的观察活化后星形胶质细胞(astrocyte,AST)的表皮生长因子受体(epithelial growth factor recep-tor,EGFR)表达变化。方法分离培养星形胶质细胞,实验分为对照组、活化组、抑制组,以睫状神经营养因子(cili-ary neurotrophic factor,CNTF)活化星形胶质细胞,并用EGFR抑制剂染料木黄酮(Genistein)干预活化星形胶质细胞,通过免疫荧光化学、RT-PCR分别观察胶原纤维酸性蛋白(glial fibrillary acidic protein,GFAP)及EGFR mRNA表达变化。结果星形胶质细胞活化后,不仅GFAP表达增高,EGFR表达亦明显增高,与对照组比较有显著差异(P0.01);应用EGFR抑制剂Genistein干预后,星形胶质细胞活化被抑制,GFAP表达下降,与活化组比较有显著差异(P0.01)。结论形胶质细胞活化后,EGFR表达明显上调;抑制EGFR表达,可抑制星形胶质细胞活化。
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| 关 键 词： | 星形胶质细胞活化 胶原纤维酸性蛋白 染料木黄酮 表皮生长因子受体 |
The relationship of the activity of astrocytes and the expression of EGFR |
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| GUO Zhi-hui,WU Heng.The relationship of the activity of astrocytes and the expression of EGFR[J].Journal of Apoplexy and Nervous Diseases,2010,27(8). |
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| Authors: | GUO Zhi-hui WU Heng |
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| Institution: | GUO Zhi-hui,WU Heng.(Department of Neurology,the First Affiliated Hospital of Nanhua University,Hengyang 421001,China) |
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| Abstract: | Objective To observe the expression of EGFR on the activivity of astrocyte in vitro.Methods Astrocytes obtained purely from newborn rats were cultured with different concentrations:normol medium,medium with ciliary neurotrophic factor(CNTF),and medium with CNTF and Genistein.After cultured for 24h,the shape and the cell cycle of astrocytes were examined by immunocytochemistry,and the expression of GFAP and EGFR were examined by RT-PCR.Results After cultured with CNTF,astrocytes were activited,the expression... |
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| Keywords: | Astrocytes GFAP Genistein EGFR |
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