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| RNAi抑制星形胶质细胞Cx43对缺氧/再复氧后细胞凋亡的影响 | |
| 引用本文： | 韩冬,冯加纯,薛晶,邓方.RNAi抑制星形胶质细胞Cx43对缺氧/再复氧后细胞凋亡的影响[J].中风与神经疾病杂志,2010,27(9). |
| 作者姓名： | 韩冬 冯加纯 薛晶 邓方 |
| 作者单位： | 1. 中国医科大学附属盛京医院神经内科,辽宁,沈阳,11004 2. 吉林大学第一医院神经内科,吉林,长春,130021 3. 吉林省医院神经内科,吉林,长春,130021 |
| 基金项目： | 国家自然科学基金资助项目(81000490); 吉林省科技发展计划项目青年基金(2010133); 吉林大学青年教师创新基金 |
| 摘 要： | 目的探讨RNA干扰技术对星形胶质细胞Cx43蛋白的抑制作用以及对细胞缺氧/再复氧后凋亡的影响。方法化学合成针对大鼠Cx43基因的小干扰RNA(Small interfering RNA,siRNA)及其阴性RNA,通过脂质体转染体外培养原代星形胶质细胞,Western blot检测Cx43蛋白表达情况,MTT法检测siRNA转染对细胞的毒性作用;通过流式细胞术观察抑制Cx43蛋白对星形胶质细胞缺氧/再复氧后细胞凋亡情况的影响。结果 Western blot结果可见,转染siRNA后12h,Cx43蛋白表达开始抑制,48h抑制最明显,MTT结果显示siRNA组和脂质体组的细胞活性明显低于未转染组(P0.05),siRNA组与脂质体组未见明显区别(P0.05);流式细胞术示缺氧12h复氧不同时间,凋亡率逐渐增加,复氧48h凋亡率最高,siRNA干扰组明显低于正常对照组(P0.05)。结论 RNAi技术是研究Cx43功能的一种有效方法,siRNA转染能有效抑制星形胶质细胞Cx43蛋白表达,siRNA转染时,细胞毒性来自脂质体;siRNA抑制Cx43蛋白能够明显减轻星形胶质细胞缺氧/再复氧后的细胞凋亡。 |
| 关 键 词： | 缝隙连接蛋白43 RNA干扰 星形胶质细胞 凋亡 |
Effect of RNA interference against Connexin43 on the apoptosis of astrocytes after hypoxia/reoxygen |
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| HAN Dong,FENG Jia-chun,XUE Jing,et al..Effect of RNA interference against Connexin43 on the apoptosis of astrocytes after hypoxia/reoxygen[J].Journal of Apoplexy and Nervous Diseases,2010,27(9). | |
| Authors: | HAN Dong FENG Jia-chun XUE Jing |
| Institution: | HAN Dong,FENG Jia-chun,XUE Jing,et al.(Department of Neurology,First Hospital of Jilin University,Changchun 130021,China) |
| Abstract: | Objective To investigate the effect of RNA interference against connexin43 of astrocytes and the influence of the apoptosis after hypoxia/reoxygen.Method Small interfering RNA(siRNA)and its negative RNA aiming to rat Cx43 gene were synthesized chemically,and transfected into astrocytes in primary culture by liposomes.Cx43 expression was detected by Western blot.The toxic effects of siRNA transfection to cells were detected by MTT.Flow cytometry was applied to detect the change of apoptosis ratio of cell hyp... |
| Keywords: | Connexin43 RNA interference Astrocyte Apoptosis |
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