甲基丙烯酸环氧丙酯对细胞间隙连接通讯的影响

为探讨甲基丙烯酸环氧丙酯（ＧＭＡ）诱导细胞转化的机理，选用划痕染料示踪技术（ＳＬＤＴ０初步研究了ＧＭＡ对人胚肺成纤维细胞间隙连接通讯（ＧＪＩＣ）的影响。用０．；５，２．５和５．０ｍｇ／Ｌ的ＧＭＡ给细胞染毒１２ｈ，经划痕导入荧光黄染料并测定各组细胞中该荧光染料向其相邻细胞扩散的程度。结果表明，在染毒剂量及时间范围内，ＧＭＡ对人胚肺成纤维细胞ＧＪＩＣ产生较强的抑制作用，呈良好的剂量－效应关系。

Effects of glycidyl methacrylate on gap junctional intercellular communication

In order to probe into the mechanism of glycidyl methacrylate (GMA) induced cell tansformation,the effects of GMA on gap junctional intercellular communication (GJIC) in human lung, embryonic fibroblasts(HLEF) were studied by using scrape loading and dye transfer(SLDT) technique.HLEF cells were treated with GMA at concentrations of 0 5,2 5 and 5 0mg/L for 12 hours and Lucifer Yellow was introduced by scrape loading after exposure.The GJIC in exposed cells and that of controls were determined directly as the diffusion extent of fluorescent dye into adjacent cells.The results indicated that GMA could inhibit the GJIC in exposed cells in a dose dependent manner.Significantly decreased GJIC was found in 2 5 and 5 0mg/L dose groups.It was suggested that the inhibition of GJIC might be one of the mechanisms responsible for GMA induced cell transformation.