戊型肝炎病人及实验感染猴粪便中戊型肝炎病毒基因的检出

采用反转录聚合酶链反应（ＲＴ－ＰＣＲ）结合ＤＮＡ杂交技术，直接检测急性戊型肝炎病人和实验感染猴粪便标中的戊型肝炎病毒（ＨＥＶ）基因，将从粪便标本中提取的ＲＮＡ反转录合成ｃＤＮＡ后，用ＨＥＶ特异引物进行套式ＰＣＲ扩增，并用长臂光敏生物素村记的ＨＥＶＥＴ１．１核酸探针进行杂交，结果显示，直接从６份病人１０％粪悬液标本中提取的ＲＮＡ进行ＲＴ－ＰＣＲ，在琼脂糖凝胶电泳上未见任何信号带，点杂交也未见阳性，但

Detection of HEV gene in fecal specimens from patients with acute hepatitis and rhesus monkeys infected with HEV 87A strain

HEV gene was detected by means of RT PCR and Southern blot assay. HEV RNA was extracted from fecal samples of six acute hepatitis patients with jaundice, and of rhesus monkeys experimentally infected by 87A strain of HEV. The cDNA was reversely transcribed and amplified by nested PCR. The RNAs and PCR products were verified by dot and Southern blot hybridization with a photosensitive biotin labeled HEV ET1.1 probe. The results showed that three of six samples precipitated by PEG were positive. In contrast, in RNA or PCR products extracted directly from fecal suspension, signals were not found except one specimen (No. 6). Using this method, the samples collected from the rhesus monkeys infected experimentally by HEV also showed to be positive. These methods are sensitive and specific for clinical diagnosis.