长期口服卡托普利对正常成年小鼠学习记忆功能的影响

目的：探讨长期口服卡托普利对正常成年小鼠学习记忆功能的影响，为卡托普利的临床应用提供实验依据。方法：选用成年小鼠，分为正常对照组及卡托普利组，卡托普利11.08～14.92 mg/kg/d 连续给药12周，通过Morris水迷宫、避暗、跳台行为学及脑胆碱系统测定，观察卡托普利对正常成年小鼠学习记忆的影响。结果：与正常对照组比较，卡托普利组小鼠第1～4天到达平台的潜伏期、游程、朝向角及游泳速度无明显改变，第5天小鼠在2 min内穿越平台的次数、在平台区的逗留时间、在平台区象限的逗留时间及平台区象限内的游程占总游程的百分比、平均速度、朝向角也无明显变化（P>0.05）；与正常对照组比较，卡托普利组小鼠第2天避暗的错误次数及错误潜伏期无明显变化（P>0.05）；与正常对照组比较，卡托普利组小鼠第1和2天跳台的错误次数及第2天的错误潜伏期无明显改变（P>0.05）；与正常对照组比较，卡托普利组小鼠脑组织乙酰胆碱（Ach）含量明显增高（P<0.01）、乙酰胆碱酯酶（AchE）含量明显升高（P<0.05）。结论：卡托普利长期应用可能提高正常成年小鼠学习记忆功能。

Effect of captopril orally administered for long term on learning and memory function of normal adult mice

Objective To explore the effect of captopril orally administered for long term on learning and memory function of normal mice in order to provide experiment base for clinical application of captopril.Methods Normal adult mice were divided control group and captopril group.The mice were administered with captopril (11.08—14.92 mg·kg-1·d-1) for 12 weeks. The effect of captopri on learning and memory was detected with Morris water maze,Avoiding dark,Step-down and cholinergic system (Ach content,AchE activity).Results Compared with control group.the latency,distance,average speed and starting angle of mice treated with captopril did not change significantly from the first day to the fourth day;the time in platform,time in platform quadrant,times passing platform ,distance in platform/total distance×100%,starting angle,and average speed also did not change significantly in the fifth day in Morris water maze (P>0.05);the error times and the latency of mice treated with captopril did not decrease in the second day in Avoiding dark(P>0.05),the error times of mice treated with captopril did not decrease in the first day and in the second day,the latency did not prolong in the second day in Step-down(P>0.05).The biochemical results showed that the content of Ach in the brain was significantly increased (P<0.01),the AchE content was also increased(P<0.05) in mice treated with captopril compared with control group.Conclusion Taking captopril for a long time may promote the function of learning and memory in normal adult mice.