菠萝叶中1,3-二氧咖啡酰甘油酯HPLC测定方法的建立

目的：建立新化合物1,3-二氧咖啡酰甘油酯HPLC测定方法,测定菠萝叶中1,3-二氧咖啡酰甘油酯的含量。方法：采用Kromasil C₁₈色谱柱(4.6 mm×150 mm,5 μm)进行分离,流动相磷酸溶液(0.1%)-乙腈（75∶25）,流速1.0 mL·min^-1,柱温30 ℃。结果：1,3-二氧咖啡酰甘油酯与其他组分分离效果较好,在0.051 6 μg～0.516 μg具有良好的线性(r=0.999 9),平均回收率为97.1%(RSD 1.3％)。经测定3批菠萝叶中1,3-二氧咖啡酰甘油酯的平均质量分数分别为0.033%,0.034%,0.031%。结论：该分析方法具有较好的分离效果以及很高的回收率和重复性,可以用于菠萝叶中1,3-二氧咖啡酰甘油酯的含量分析及质量控制。

Determination of 1,3-O-dicaffeoylglycerides from leaves of pineapple by HPLC

OBJECTIVE: To establish a HPLC method for a new compound 1,3-O-dicaffeoylglycerides determination. METHOD: The separation was performed in a Kromasil C18 column (4.6 mm x 150 mm, 5 microm) with a mobile phase of 0.1% H3PO4: Acetontrile = 75 : 25 (v/v). The flow rate was 1.0 ml x min(-)1 and the temperature of column was 30 degrees C. RESULT: A satisfactory separation between 1, 3-O-dicaffeoylglycerides and impurity was obtained. The calibration curve was linear over the concentration range from 0.051 6 microg to 0.516 microg, r = 0.999 9. The average recoveries was 97.1% (RSD 1.3%). The content of 1,3-O-dicaffeoylglycerides in pineapple leaves from three different batches were 0.033%, 0.034% and 0.031% respectively. CONCLUSION: The method has good selectivity, high recovery and reproducibility, and can be used for the analysis of 1,3-O-dicaffeoylglycerides in pineapple leaves and their quality control.