环孢素A对神经肽Y刺激的大鼠血管平滑肌细胞增殖的影响

目的:观察环孢素A(CsA)对神经肽Y(NPY)诱导的大鼠主动脉平滑肌细胞增殖的影响,以探讨钙调神经磷酸酶(CaN)信号通路在血管平滑肌细胞增殖中的作用。方法:体外培养的大鼠主动脉平滑肌细胞分为3组:(1)NPY组,(2)CsA+NPY组,(3)对照组。检测CaN活性(定磷法)、细胞增殖活度(MTT法)的变化,观察增殖细胞核抗原(PCNA)的表达水平(免疫组化定量技术)。结果:NPY组CaN活性、血管平滑肌细胞增殖活度(吸光度表示),PCNA表达水平(光密度值表示)明显高于对照组(P＜0.01或P＜0.05),CsA+NPY组各项指标明显低于NPY组(P＜0.01或P＜0.05)。结论:环孢素A可显著阻滞神经肽Y刺激的血管平滑肌细胞增殖,这种作用可能通过抑制CaN信号通路所致。

Effect of cyclosporin A on neuropeptide Y-induced proliferation of cultured vascular smooth muscle cells

AIM: To study the role of calcineurin (CaN)-dependent signaling pathway in proliferation of vascular smooth muscle cells (VSMCs) by observing the effect of cyclosporin A (CsA) on proliferation of neuropeptide Y (NPY)-induced rat VSMCs. METHODS: Upon the model of cultured rat VSMCs, the study consisted of three groups: NPYgroup,CsA+NPY group and control group. CaN activity was determinated by enzyme reaction phosphorus measurement. The methods of biochemistry (MTT) and quantitative immunocytochemistry were applied to investigate the proliferation of VSMC and the expression of proliferation cell nuclear antigen (PCNA) in cultured rat VSMCs. RESULTS: Compared with the control group, the VSMC's CaN activity, proliferation activity and expression of PCNA (by photo densitometry A_PCNA) were obviously increased in NPY group (P＜0.01 or P＜0.05). Meanwhile, CsA markedly inhibited CaN activity , proliferation activity and the expression of PCNA with a significant difference from NPY group (P＜0.01 or P＜0.05). CONCLUSION: The study indicates that CsA has a significant inhibitory effect on proliferation of VSMC stimulated by NPY, which may be related to blocking CaN-mediated signal transduction.